Lactulose production from lactose isomerization by chemo-catalysts and enzymes: Current status and future perspectives

M Wang, L Wang, X Lyu, X Hua, JM Goddard… - Biotechnology …, 2022 - Elsevier
Lactulose, a semisynthetic nondigestive disaccharide with versatile applications in the food
and pharmaceutical industries, has received increasing interest due to its significant health …

[HTML][HTML] Generative artificial intelligence for de novo protein design

A Winnifrith, C Outeiral, BL Hie - Current Opinion in Structural Biology, 2024 - Elsevier
Engineering new molecules with desirable functions and properties has the potential to
extend our ability to engineer proteins beyond what nature has so far evolved. Advances in …

Design of artificial enzymes bearing several active centers: New trends, opportunities and problems

D Carballares, R Morellon-Sterling… - International Journal of …, 2022 - mdpi.com
Harnessing enzymes which possess several catalytic activities is a topic where intense
research has been carried out, mainly coupled with the development of cascade reactions …

Cu-MOF@ Pt 3D nanocomposites prepared by one-step wrapping method with peroxidase-like activity for colorimetric detection of glucose

R Zhou, X Zhuang, Q Wu, M Jin, C Zheng… - Colloids and Surfaces B …, 2022 - Elsevier
As an alternative to natural enzymes, artificial enzymes based on nanomaterials have
attracted a lot of attention owing to their outstanding catalytic activity and high stability as …

QM/MM Modeling Aided Enzyme Engineering in Natural Products Biosynthesis

F Zhang, T Zeng, R Wu - Journal of Chemical Information and …, 2023 - ACS Publications
Natural products and their derivatives are widely used across various industries, particularly
pharmaceuticals. Modern engineered biosynthesis provides an alternative way of producing …

微液滴高通量筛选方法的研究与应用进展

秦伟彤, 杨广宇 - 合成生物学, 2023 - synbioj.cip.com.cn
在单细胞层面对生物功能进行高通量的分析和分选是对关键基因, 元件, 途径与细胞工厂进行
优化的重要技术. 基于微液滴的筛选方法因其低成本, 超高通量等优势, 已被广泛应用于生物 …

Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations

HM Huttanus, EKH Triola… - … in Bioengineering and …, 2023 - frontiersin.org
Targeted mutagenesis of a promoter or gene is essential for attaining new functions in
microbial and protein engineering efforts. In the burgeoning field of synthetic biology …

Acid-resistant enzymes: the acquisition strategies and applications

Z Zhang, Z Zhao, K Huang, Z Liang - Applied Microbiology and …, 2023 - Springer
Enzymes have promising applications in chemicals, food, pharmaceuticals, and other variety
products because of their high efficiency, specificity, and environmentally friendly properties …

Improving Thermostability and Catalytic Activity of Glycosyltransferase From Panax ginseng by Semi-Rational Design for Rebaudioside D Synthesis

M Chen, F Song, Y Qin, S Han, Y Rao… - … in Bioengineering and …, 2022 - frontiersin.org
As a natural sweetener and sucrose substitute, the biosynthesis and application of steviol
glycosides containing the component rebaudioside D have attracted worldwide attention …

Dual-Enzyme Cascade Composed of Chitosan Coated FeS2 Nanozyme and Glucose Oxidase for Sensitive Glucose Detection

B Shen, M Qing, L Zhu, Y Wang, L Jiang - Molecules, 2023 - mdpi.com
Immobilizing enzymes with nanozymes to catalyze cascade reactions overcomes many of
the shortcomings of biological enzymes in industrial manufacturing. In the study, glucose …