[HTML][HTML] Engineering organoids
Organoids are in vitro miniaturized and simplified model systems of organs that have gained
enormous interest for modelling tissue development and disease, and for personalized …
enormous interest for modelling tissue development and disease, and for personalized …
Drug delivery systems for CRISPR-based genome editors
V Madigan, F Zhang, JE Dahlman - Nature Reviews Drug Discovery, 2023 - nature.com
CRISPR-based drugs can theoretically manipulate any genetic target. In practice, however,
these drugs must enter the desired cell without eliciting an unwanted immune response, so …
these drugs must enter the desired cell without eliciting an unwanted immune response, so …
The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases
IscB proteins are putative nucleases encoded in a distinct family of IS200/IS605 transposons
and are likely ancestors of the RNA-guided endonuclease Cas9, but the functions of IscB …
and are likely ancestors of the RNA-guided endonuclease Cas9, but the functions of IscB …
[HTML][HTML] Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing
Compact and versatile CRISPR-Cas systems will enable genome engineering applications
through high-efficiency delivery in a wide variety of contexts. Here, we create an efficient …
through high-efficiency delivery in a wide variety of contexts. Here, we create an efficient …
Lipid nanoparticle-mediated codelivery of Cas9 mRNA and single-guide RNA achieves liver-specific in vivo genome editing of Angptl3
Loss-of-function mutations in Angiopoietin-like 3 (Angptl3) are associated with lowered
blood lipid levels, making Angptl3 an attractive therapeutic target for the treatment of human …
blood lipid levels, making Angptl3 an attractive therapeutic target for the treatment of human …
The CRISPR–Cas toolbox for analytical and diagnostic assay development
Y Tang, L Gao, W Feng, C Guo, Q Yang, F Li… - Chemical Society …, 2021 - pubs.rsc.org
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
[HTML][HTML] Adeno-associated virus toolkit to target diverse brain cells
RC Challis, S Ravindra Kumar, X Chen… - Annual review of …, 2022 - annualreviews.org
Recombinant adeno-associated viruses (AAVs) are commonly used gene delivery vehicles
for neuroscience research. They have two engineerable features: the capsid (outer protein …
for neuroscience research. They have two engineerable features: the capsid (outer protein …
CRISPR technologies for genome, epigenome and transcriptome editing
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …
understanding of CRISPR biology and its application to genome, epigenome and …
CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
[HTML][HTML] CRISPR-based therapeutic genome editing: strategies and in vivo delivery by AAV vectors
The development of clustered regularly interspaced short-palindromic repeat (CRISPR)-
based biotechnologies has revolutionized the life sciences and introduced new therapeutic …
based biotechnologies has revolutionized the life sciences and introduced new therapeutic …