Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

Environmental challenges related to the mismanagement of plastic waste became even
more evident during the COVID-19 pandemic. The need for new solutions regarding the use …

Mixed plastics waste represents an abundant and largely untapped feedstock for the
production of valuable products. The chemical diversity and complexity of these materials …

Plastic pollution is rapidly increasing worldwide, causing adverse impacts on the
environment, wildlife and human health. One tempting solution to this crisis is upcycling …

CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

The runaway production and consumption of oil-based plastics are key drivers of global
warming and the increased carbon footprint. Besides, most of this plastic debris ends up in …

Formate is a promising, water-soluble C1 feedstock for biotechnology that can be efficiently
produced from CO 2—but formatotrophy has been engineered in only a few industrially …

Major advances toward a bio-based industry enabled cost-efficient bioproduction of multiple
chemicals, yet successful industrial processes are relatively scarce and limited to the use of …

Pseudomonas putida is evolutionarily endowed with features relevant for bioproduction,
especially under harsh operating conditions. The rich metabolic versatility of this species …

In light of the current concerns about environmental issues caused by the excessive use of
fossil resources, more emphasis has been paid to the transition to a sustainable and circular …