CRISPR/Cas9 advances engineering of microbial cell factories

T Jakočiūnas, MK Jensen, JD Keasling - Metabolic engineering, 2016 - Elsevier
One of the key drivers for successful metabolic engineering in microbes is the efficacy by
which genomes can be edited. As such there are many methods to choose from when …

Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates

MP Mezzina, MT Manoli, MA Prieto… - Biotechnology …, 2021 - Wiley Online Library
Growing environmental concern sparks renewed interest in the sustainable production of
(bio) materials that can replace oil‐derived goods. Polyhydroxyalkanoates (PHAs) are …

Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain

M Florea, H Hagemann, G Santosa… - Proceedings of the …, 2016 - National Acad Sciences
Bacterial cellulose is a strong and ultrapure form of cellulose produced naturally by several
species of the Acetobacteraceae. Its high strength, purity, and biocompatibility make it of …

Low carbon strategies for sustainable bio-alkane gas production and renewable energy

M Amer, EZ Wojcik, C Sun, R Hoeven… - Energy & …, 2020 - pubs.rsc.org
Propane and butane are the main constituents of liquefied petroleum gas and are used
extensively for transport and domestic use. They are clean burning fuels, suitable for the …

Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression

E Martínez-García, PI Nikel, T Aparicio… - Microbial cell …, 2014 - Springer
Background Because of its adaptability to sites polluted with toxic chemicals, the model soil
bacterium Pseudomonas putida is naturally endowed with a number of metabolic and stress …

[HTML][HTML] Automating the design-build-test-learn cycle towards next-generation bacterial cell factories

N Gurdo, DC Volke, D McCloskey, PI Nikel - New Biotechnology, 2023 - Elsevier
Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

A fluoride-responsive genetic circuit enables in vivo biofluorination in engineered Pseudomonas putida

P Calero, DC Volke, PT Lowe, CH Gotfredsen… - Nature …, 2020 - nature.com
Fluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture
and materials. Addition of fluorine to organic structures represents a unique strategy for …

[HTML][HTML] A navigation guide of synthetic biology tools for Pseudomonas putida

M Martin-Pascual, C Batianis, L Bruinsma… - Biotechnology …, 2021 - Elsevier
Pseudomonas putida is a microbial chassis of huge potential for industrial and
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …

CyanoGate: a modular cloning suite for engineering cyanobacteria based on the plant MoClo syntax

R Vasudevan, GAR Gale, AA Schiavon… - Plant …, 2019 - academic.oup.com
Recent advances in synthetic biology research have been underpinned by an exponential
increase in available genomic information and a proliferation of advanced DNA assembly …

SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities

E Martínez-García, T Aparicio… - Nucleic acids …, 2015 - academic.oup.com
Abstract The Standard European Vector Architecture 2.0 database (SEVA-DB 2.0,
http://seva. cnb. csic. es) is an improved and expanded version of the platform released in …