Extremophiles are suitable chassis for developing the next generation industrial
biotechnology (NGIB) due to their resistance to microbial contamination. However …

Pseudomonas putida is a promising bacterial host for producing natural products, such as
polyketides and nonribosomal peptides. In these types of projects, researchers need a …

Background Plasmids are widely used for molecular cloning or production of proteins in
laboratory and industrial settings. Constant modification has brought forth countless plasmid …

Industrial lignin such as kraft lignin is an abundant feedstock for renewable chemicals and
materials. In this study, a process was developed for depolymerization of kraft lignin followed …

Background The implementation of novel platform organisms to be used as microbial cell
factories in industrial applications is currently the subject of intense research. Ongoing …

Pseudomonas putida is evolutionarily endowed with features relevant for bioproduction,
especially under harsh operating conditions. The rich metabolic versatility of this species …

Genome engineering of non-conventional microorganisms calls for the development of
dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic …

In this work we report synthetic adhesins (SAs) enabling the rational design of the adhesion
properties of E. coli. SAs have a modular structure comprising a stable β-domain for outer …

Methods for simple and efficient regulation of metabolic pathway genes are essential for
maximizing product titers and conversion yields, and for minimizing the metabolic burden …

Given its capacity to tolerate stress, NAD (P) H/NAD (P) balance, and increased ATP levels,
the platform strain Pseudomonas putida EM42, a genome-edited derivative of the soil …