An expanded CRISPR–Cas9-assisted recombineering toolkit for engineering genetically intractable Pseudomonas aeruginosa isolates
D Pankratz, NO Gomez, A Nielsen, A Mustafayeva… - Nature protocols, 2023 - nature.com
Much of our current understanding of microbiology is based on the application of genetic
engineering procedures. Since their inception (more than 30 years ago), methods based …
engineering procedures. Since their inception (more than 30 years ago), methods based …
CRISPR interference-guided balancing of a biosynthetic mevalonate pathway increases terpenoid production
Methods for simple and efficient regulation of metabolic pathway genes are essential for
maximizing product titers and conversion yields, and for minimizing the metabolic burden …
maximizing product titers and conversion yields, and for minimizing the metabolic burden …
[HTML][HTML] Refactoring the upper sugar metabolism of Pseudomonas putida for co-utilization of cellobiose, xylose, and glucose
P Dvořák, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
Given its capacity to tolerate stress, NAD (P) H/NAD (P) balance, and increased ATP levels,
the platform strain Pseudomonas putida EM42, a genome-edited derivative of the soil …
the platform strain Pseudomonas putida EM42, a genome-edited derivative of the soil …
Dynamic allocation of orthogonal ribosomes facilitates uncoupling of co-expressed genes
Introduction of synthetic circuits into microbes creates competition between circuit and host
genes for shared cellular resources, such as ribosomes. This can lead to the emergence of …
genes for shared cellular resources, such as ribosomes. This can lead to the emergence of …
Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria
Non-model bacteria like Pseudomonas putida, Lactococcus lactis and other species have
unique and versatile metabolisms, offering unique opportunities for Synthetic Biology …
unique and versatile metabolisms, offering unique opportunities for Synthetic Biology …
Muconic Acid Production Using Engineered Pseudomonas putida KT2440 and a Guaiacol-Rich Fraction Derived from Kraft Lignin
H Almqvist, H Veras, K Li… - ACS Sustainable …, 2021 - ACS Publications
Industrial lignin such as kraft lignin is an abundant feedstock for renewable chemicals and
materials. In this study, a process was developed for depolymerization of kraft lignin followed …
materials. In this study, a process was developed for depolymerization of kraft lignin followed …
Chromosome engineering of the TCA cycle in Halomonas bluephagenesis for production of copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate (PHBV)
Abstract Poly (3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV) is a promising biopolyester
with good mechanical properties and biodegradability. Large-scale production of PHBV is …
with good mechanical properties and biodegradability. Large-scale production of PHBV is …
Promoter Engineering for Enhanced P(3HB-co-4HB) Production by Halomonas bluephagenesis
Promoters for the expression of heterologous genes in Halomonas bluephagenesis are
quite limited, and many heterologous promoters function abnormally in this strain. Pporin, a …
quite limited, and many heterologous promoters function abnormally in this strain. Pporin, a …
Engineering Halomonas bluephagenesis TD01 for non-sterile production of poly (3-hydroxybutyrate-co-4-hydroxybutyrate)
Abstract Poly (3-hydroxybutyrate-co-4-hydroxybutyrate), short as P (3HB-co-4HB), was
successfully produced by engineered Halomonas bluephagenesis TD01 grown in glucose …
successfully produced by engineered Halomonas bluephagenesis TD01 grown in glucose …
[HTML][HTML] Synthetic control of plasmid replication enables target-and self-curing of vectors and expedites genome engineering of Pseudomonas putida
Genome engineering of non-conventional microorganisms calls for the development of
dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic …
dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic …