Biosynthesis of functional polyhydroxyalkanoates by engineered Halomonas bluephagenesis
Polyhydroxyalkanoates (PHA) have found widespread medical applications due to their
biocompatibility and biodegradability, while further chemical modification requires functional …
biocompatibility and biodegradability, while further chemical modification requires functional …
Absolute protein quantification using fluorescence measurements with FPCountR
This paper presents a generalisable method for the calibration of fluorescence readings on
microplate readers, in order to convert arbitrary fluorescence units into absolute units …
microplate readers, in order to convert arbitrary fluorescence units into absolute units …
Systematic evasion of the restriction-modification barrier in bacteria
CD Johnston, SL Cotton, SR Rittling… - Proceedings of the …, 2019 - National Acad Sciences
Bacteria that are recalcitrant to genetic manipulation using modern in vitro techniques are
termed genetically intractable. Genetic intractability is a fundamental barrier to progress that …
termed genetically intractable. Genetic intractability is a fundamental barrier to progress that …
Tailor‐Made Polyhydroxyalkanoates by Reconstructing Pseudomonas Entomophila
Microbial polyhydroxyalkanoates (PHA) containing short‐and medium/long‐chain‐length
monomers, abbreviated as SCL‐co‐MCL/LCL PHAs, generate suitable thermal and …
monomers, abbreviated as SCL‐co‐MCL/LCL PHAs, generate suitable thermal and …
Role of the CrcB transporter of Pseudomonas putida in the multi‐level stress response elicited by mineral fluoride
The presence of mineral fluoride (F−) in the environment has both a geogenic and
anthropogenic origin, and the halide has been described to be toxic in virtually all living …
anthropogenic origin, and the halide has been described to be toxic in virtually all living …
A plasmid toolbox for controlled gene expression across the Proteobacteria
LA Schuster, CR Reisch - Nucleic Acids Research, 2021 - academic.oup.com
Controlled gene expression is fundamental for the study of gene function and our ability to
engineer bacteria. However, there is currently no easy-to-use genetics toolbox that enables …
engineer bacteria. However, there is currently no easy-to-use genetics toolbox that enables …
[HTML][HTML] Creating metabolic demand as an engineering strategy in Pseudomonas putida–Rhamnolipid synthesis as an example
T Tiso, P Sabelhaus, B Behrens, A Wittgens… - Metabolic Engineering …, 2016 - Elsevier
Metabolic engineering of microbial cell factories for the production of heterologous
secondary metabolites implicitly relies on the intensification of intracellular flux directed …
secondary metabolites implicitly relies on the intensification of intracellular flux directed …
Engineering of Pseudomonas putida for accelerated co-utilization of glucose and cellobiose yields aerobic overproduction of pyruvate explained by an upgraded …
Pseudomonas putida KT2440 is an attractive bacterial host for biotechnological production
of valuable chemicals from renewable lignocellulosic feedstocks as it can valorize lignin …
of valuable chemicals from renewable lignocellulosic feedstocks as it can valorize lignin …
Developments in the tools and methodologies of synthetic biology
Synthetic biology is principally concerned with the rational design and engineering of
biologically based parts, devices, or systems. However, biological systems are generally …
biologically based parts, devices, or systems. However, biological systems are generally …
Broad-Host-Range ProUSER Vectors Enable Fast Characterization of Inducible Promoters and Optimization of p-Coumaric Acid Production in Pseudomonas putida …
P Calero, SI Jensen, AT Nielsen - ACS synthetic biology, 2016 - ACS Publications
Pseudomonas putida KT2440 has gained increasing interest as a host for the production of
biochemicals. Because of the lack of a systematic characterization of inducible promoters in …
biochemicals. Because of the lack of a systematic characterization of inducible promoters in …