Reversible phosphorylation of Pol II and accessory factors helps order the transcription
cycle. Here, we define two kinase-phosphatase switches that operate at different points in …

The genomes of kinetoplastids are organized into polycistronic transcription units that are
flanked by a modified DNA base (base J, beta-D-glucosyl-hydroxymethyluracil). Previous …

Background The sequence content of the 3′ UTRs of many mRNA transcripts is regulated
through alternative polyadenylation (APA). The study of this process using RNAseq data …

Controlled release of promoter-proximal paused RNA polymerase II (RNA Pol II) is crucial
for gene regulation. However, studying RNA Pol II pausing is challenging, as pause-release …

The mechanisms and timescales controlling de novo establishment of chromatin-mediated
transcriptional silencing by Polycomb repressive complex 2 (PRC2) are unclear. Here, we …

Interactions between the splicing machinery and RNA polymerase II increase protein-coding
gene transcription. Similarly, exons and splicing signals of enhancer-generated long …

Transcription elongation by RNA polymerase II (Pol II) has emerged as a regulatory hub in
gene expression. A key control point occurs during early transcription elongation when Pol II …

The transcription cycle of RNA polymerase II (RNAPII) is governed at multiple points by
opposing actions of cyclin-dependent kinases (CDKs) and protein phosphatases, in a …

The interconnections between co-transcriptional regulation, chromatin environment, and
transcriptional output remain poorly understood. Here, we investigate the mechanism …

The human genome encodes thousands of non-coding RNAs. Many of these terminate early
and are then rapidly degraded, but how their transcription is restricted is poorly understood …