[HTML][HTML] Pseudomonas putida as a functional chassis for industrial biocatalysis: from native biochemistry to trans-metabolism

PI Nikel, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer
bacterium to become a flexible and engineer-able platform for metabolic engineering stems …

Gene-expression tools for the metabolic engineering of bacteria

JD Keasling - Trends in biotechnology, 1999 - cell.com
Recent advances in metabolic engineering have led to new methods for the synthesis of
novel molecules, improved production of existing compounds and improved degradation of …

PhaF, a Polyhydroxyalkanoate-Granule-Associated Protein of Pseudomonas oleovorans GPo1 Involved in the Regulatory Expression System for pha Genes

MA Prieto, B Bühler, K Jung, B Witholt… - Journal of …, 1999 - Am Soc Microbiol
The phaC1 gene codes for the medium-chain-length polyhydroxyalkanoate (mcl PHA)
synthase of Pseudomonas oleovorans GPo1, which produces mcl PHA when grown in an …

Getting bacteria in shape: synthetic morphology approaches for the design of efficient microbial cell factories

DC Volke, PI Nikel - Advanced Biosystems, 2018 - Wiley Online Library
Supported by the tools of contemporary synthetic biology, the field of metabolic engineering
has advanced in its overarching purpose of contributing efficient bioprocesses for the …

Resistance to Tellurite as a Selection Marker for Genetic Manipulations of Pseudomonas Strains

JM Sanchez-Romero, R Diaz-Orejas… - Applied and …, 1998 - Am Soc Microbiol
Resistance to the toxic compound potassium tellurite (Telr) has been employed as a
selection marker built into a set of transposon vectors and broad-host-range plasmids …

Characterization of the hca Cluster Encoding the Dioxygenolytic Pathway for Initial Catabolism of 3-Phenylpropionic Acid in Escherichia coliK-12

E Díaz, A Ferrández, JL García - Journal of bacteriology, 1998 - Am Soc Microbiol
We have identified, cloned, and sequenced the hca cluster encoding the dioxygenolytic
pathway for initial catabolism of 3-phenylpropionic acid (PP) in Escherichia coli K-12. This …

Role of the crc Gene in Catabolic Repression of the Pseudomonas putida GPo1 Alkane Degradation Pathway

L Yuste, F Rojo - Journal of bacteriology, 2001 - Am Soc Microbiol
Expression of the alkane degradation pathway encoded in the OCT plasmid of
Pseudomonas putida GPo1 is induced in the presence of alkanes by the AlkS regulator, and …

Mini-transposons in microbial ecology and environmental biotechnology

V de Lorenzo, M Herrero, JM Sánchez… - FEMS Microbiology …, 1998 - academic.oup.com
Mini-transposon is the generic name given to the members of a collection of genetic assets
derived from transposons Tn 10 and Tn 5, in which the naturally occurring functional …

A dual lethal system to enhance containment of recombinant micro-organisms

B Torres, S Jaenecke, KN Timmis, JL García… - …, 2003 - microbiologyresearch.org
Active containment systems based on the controlled expression of a lethal gene are
designed to increase containment of recombinant micro-organisms used for environmental …

Current Status of Pseudomonas putida Engineering for Lignin Valorization

S Lee, JH Sohn, JH Bae, SC Kim, BH Sung - … and Bioprocess Engineering, 2020 - Springer
Lignin, a complex aromatic polymer, is a structural component of plant biomass. It
decomposes with difficulty because of its rigidity properties, however, lignin valorization is …