The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …

CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

Targeted mutagenesis via genome‐editing technologies holds great promise in developing
improved crop varieties to meet future demands. Point mutations or single nucleotide …

Prime-editing systems have the capability to perform efficient and precise genome editing in
human cells. In this study, we first developed a plant prime editor 2 (pPE2) system and test …

CRISPR/Cas9 genome editing relies on sgRNA-target DNA base pairing and a short
downstream PAM sequence to recognize target DNA. The strict protospacer adjacent motif …

Cytosine base editors (CBEs) are great additions to the expanding genome editing toolbox.
To improve C‐to‐T base editing in plants, we first compared seven cytidine deaminases in …

Two recently engineered SpCas9 variants, namely xCas9 and Cas9-NG, show promising
potential in improving targeting specificity and broadening the targeting range. In this study …

Environmental abiotic stresses challenge food security by depressing crop yields often
exceeding 50% of their annual production. Different methods, including conventional as well …

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated
protein (Cas)-mediated gene disruption has revolutionized biomedical research as well as …

Adenine base editors (ABE s) have been exploited to introduce targeted adenine (A) to
guanine (G) base conversions in various plant genomes, including rice, wheat and …