CRISPR-based genome editing tools: Insights into technological breakthroughs and future challenges

M Mushtaq, A Ahmad Dar, M Skalicky, A Tyagi… - Genes, 2021 - mdpi.com
Genome-editing (GE) is having a tremendous influence around the globe in the life science
community. Among its versatile uses, the desired modifications of genes, and more …

[HTML][HTML] Targeted base editing in rice with CRISPR/ScCas9 system

M Wang, Z Xu, G Gosavi, B Ren, Y Cao… - Plant Biotechnology …, 2020 - ncbi.nlm.nih.gov
The CRISPR/Cas system has rapidly become the preferred tool for genome engineering in
various organisms due to high efficiency, specificity, simplicity and versatility. Currently …

碱基编辑系统研究进展

宗媛, 高彩霞 - 遗传, 2019 - chinagene.cn
碱基编辑技术(base editing) 是基于CRISPR/Cas 系统发展起来的新型靶基因修饰技术,
目前依据碱基修饰酶的不同可分为胞嘧啶碱基编辑器(cytosine base editor, CBE) …

Genome editing technologies for rice improvement: progress, prospects, and safety concerns

K Zafar, KEM Sedeek, GS Rao, MZ Khan… - Frontiers in Genome …, 2020 - frontiersin.org
Rice (Oryza sativa) is an important staple food crop worldwide; to meet the growing
nutritional requirements of the increasing population in the face of climate change …

Applications and prospects of CRISPR/Cas9-mediated base editing in plant breeding

Y Li, J Liang, B Deng, Y Jiang, J Zhu, L Chen… - Current Issues in …, 2023 - mdpi.com
The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein
9 system (Cas9) has been used at length to optimize multiple aspects of germplasm …

Plant genome editing: ever more precise and wide reaching

S Sukegawa, H Saika, S Toki - The Plant Journal, 2021 - Wiley Online Library
Genome‐editing technologies consisting of targeted mutagenesis and gene targeting
enable us to modify genes of interest rapidly and precisely. The discovery in 2012 of …

Precision breeding made real with CRISPR: illustration through genetic resistance to pathogens

F Veillet, M Durand, T Kroj, S Cesari, JL Gallois - Plant Communications, 2020 - cell.com
Since its discovery as a bacterial adaptive immune system and its development for genome
editing in eukaryotes, the CRISPR technology has revolutionized plant research and …

Base editing: The ever expanding clustered regularly interspaced short palindromic repeats (CRISPR) tool kit for precise genome editing in plants

MB Monsur, G Shao, Y Lv, S Ahmad, X Wei, P Hu… - Genes, 2020 - mdpi.com
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated
protein 9 (Cas9), a newly developed genome-editing tool, has revolutionized animal and …

[HTML][HTML] SpCas9-NG self-targets the sgRNA sequence in plant genome editing

R Qin, J Li, X Liu, R Xu, J Yang, P Wei - Nature Plants, 2020 - nature.com
Abstract Streptococcus pyogenes Cas9 (SpCas9)-NG recognizes NGN protospacer
adjacent motifs and expands the scope of genome-editing tools. In this study, we found that …

[HTML][HTML] Genome editing technology and application in soybean improvement

A Bao, C Zhang, Y Huang, H Chen, X Zhou, D Cao - Oil Crop Science, 2020 - Elsevier
Soybean (Glycine max) is a legume crop with great economic value that provides rich
protein and oil for human food and animal feed. In order to cope with the ever-increasing …