RNA–protein interactions underlie a wide range of cellular processes. Improved methods
are needed to systematically map RNA–protein interactions in living cells in an unbiased …

Characterizing the proteome composition of organelles and subcellular regions of living
cells can facilitate the understanding of cellular organization as well as protein interactome …

Regulation of gene expression is primarily controlled by changes in the proteins that occupy
genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we …

Conspectus Proximity labeling can be defined as an enzymatic “in-cell” chemical reaction
that catalyzes the proximity-dependent modification of biomolecules in live cells. Since the …

APEX is an engineered peroxidase that catalyzes the oxidation of a wide range of
substrates, facilitating its use in a variety of applications from subcellular staining for electron …

Each heartbeat is initiated by the action potential, an electrical signal that depolarizes the
plasma membrane and activates a cycle of calcium influx via voltage-gated calcium …

Protein analysis under biological conditions is now regarded as indispensable for
understanding the structure and function of proteins, in addition to in vitro studies using …

The spatial organization of RNA within cells is a crucial factor influencing a wide range of
biological functions throughout all kingdoms of life. However, a general understanding of …

The primary cilium is a signaling compartment that interprets Hedgehog signals through
changes of its protein, lipid, and second messenger compositions. Here, we combine …

Enzymatic-based proximity labeling approaches based on activated esters or phenoxy
radicals have been widely used for mapping subcellular proteome and protein interactors in …