[HTML][HTML] Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

[HTML][HTML] Pseudomonas putida as a functional chassis for industrial biocatalysis: from native biochemistry to trans-metabolism

PI Nikel, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer
bacterium to become a flexible and engineer-able platform for metabolic engineering stems …

Bioremediation 3.0: engineering pollutant-removing bacteria in the times of systemic biology

P Dvořák, PI Nikel, J Damborský, V de Lorenzo - Biotechnology advances, 2017 - Elsevier
Elimination or mitigation of the toxic effects of chemical waste released to the environment
by industrial and urban activities relies largely on the catalytic activities of microorganisms …

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

P Calero, PI Nikel - Microbial Biotechnology, 2019 - Wiley Online Library
The last few years have witnessed an unprecedented increase in the number of novel
bacterial species that hold potential to be used for metabolic engineering. Historically …

The revisited genome of Pseudomonas putida KT2440 enlightens its value as a robust metabolic chassis

E Belda, RGA Van Heck… - Environmental …, 2016 - Wiley Online Library
By the time the complete genome sequence of the soil bacterium Pseudomonas putida
KT2440 was published in 2002 (Nelson et al.,) this bacterium was considered a potential …

[HTML][HTML] Pseudomonas putida KT2440 strain metabolizes glucose through a cycle formed by enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and …

PI Nikel, M Chavarría, T Fuhrer, U Sauer… - Journal of Biological …, 2015 - ASBMB
The soil bacterium Pseudomonas putida KT2440 lacks a functional Embden-Meyerhof-
Parnas (EMP) pathway, and glycolysis is known to proceed almost exclusively through the …

[HTML][HTML] Systems biology of microbial exopolysaccharides production

O Ates - Frontiers in bioengineering and biotechnology, 2015 - frontiersin.org
Exopolysaccharides (EPSs) produced by diverse group of microbial systems are rapidly
emerging as new and industrially important biomaterials. Due to their unique and complex …

Biotechnological domestication of pseudomonads using synthetic biology

PI Nikel, E Martínez-García, V De Lorenzo - Nature Reviews …, 2014 - nature.com
Much of contemporary synthetic biology research relies on the use of bacterial chassis for
plugging-in and plugging-out genetic circuits and new-to-nature functionalities. However …

High‐quality genome‐scale metabolic modelling of Pseudomonas putida highlights its broad metabolic capabilities

J Nogales, J Mueller, S Gudmundsson… - Environmental …, 2020 - Wiley Online Library
Genome‐scale reconstructions of metabolism are computational species‐specific
knowledge bases able to compute systemic metabolic properties. We present a …

Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates

MP Mezzina, MT Manoli, MA Prieto… - Biotechnology …, 2021 - Wiley Online Library
Growing environmental concern sparks renewed interest in the sustainable production of
(bio) materials that can replace oil‐derived goods. Polyhydroxyalkanoates (PHAs) are …