[HTML][HTML] New photoacids in microarray synthesis of oligonucleotides
AN Sinyakov, EV Kostina, DE Zaytsev… - Journal of Saudi …, 2023 - Elsevier
Photochemical activity of thiophene oxime esters and of sulfonium salt tris [4-(4-
acetylphenyl) sulfanylphenyl] sulfonium tetrakis (2, 3, 4, 5, 6-pentafluorophenyl) boranuide …
acetylphenyl) sulfanylphenyl] sulfonium tetrakis (2, 3, 4, 5, 6-pentafluorophenyl) boranuide …
[HTML][HTML] A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast
NJ McGlincy, ZA Meacham, KK Reynaud, R Muller… - BMC genomics, 2021 - Springer
Background CRISPR/Cas9-mediated transcriptional interference (CRISPRi) enables
programmable gene knock-down, yielding loss-of-function phenotypes for nearly any gene …
programmable gene knock-down, yielding loss-of-function phenotypes for nearly any gene …
[HTML][HTML] A scalable double-barcode sequencing platform for characterization of dynamic protein-protein interactions
Several large-scale efforts have systematically catalogued protein-protein interactions (PPIs)
of a cell in a single environment. However, little is known about how the protein interactome …
of a cell in a single environment. However, little is known about how the protein interactome …
Improved discovery of genetic interactions using CRISPRiSeq across multiple environments
Large-scale genetic interaction (GI) screens in yeast have been invaluable for our
understanding of molecular systems biology and for characterizing novel gene function …
understanding of molecular systems biology and for characterizing novel gene function …
Quantitative analysis of protein interaction network dynamics in yeast
Many cellular functions are mediated by protein–protein interaction networks, which are
environment dependent. However, systematic measurement of interactions in diverse …
environment dependent. However, systematic measurement of interactions in diverse …
[HTML][HTML] Yeast genetic interaction screens in the age of CRISPR/Cas
The ease of performing both forward and reverse genetics in Saccharomyces cerevisiae,
along with its stable haploid state and short generation times, has made this budding yeast …
along with its stable haploid state and short generation times, has made this budding yeast …
A CRISPR interference screen of essential genes reveals that proteasome regulation dictates acetic acid tolerance in Saccharomyces cerevisiae
ABSTRACT CRISPR interference (CRISPRi) is a powerful tool to study cellular physiology
under different growth conditions, and this technology provides a means for screening …
under different growth conditions, and this technology provides a means for screening …
[HTML][HTML] Identification of acetic acid sensitive strains through biosensor-based screening of a Saccharomyces cerevisiae CRISPRi library
Background Acetic acid tolerance is crucial for the development of robust cell factories for
conversion of lignocellulosic hydrolysates that typically contain high levels of acetic acid …
conversion of lignocellulosic hydrolysates that typically contain high levels of acetic acid …
Molecular tools for pathway engineering in Saccharomyces cerevisiae
PB Besada-Lombana, TL McTaggart… - Current opinion in …, 2018 - Elsevier
Highlights•Advances in CRISPR-Cas9 aid tool development and multiplexed yeast
engineering.•New catalogue of promoters, trans-acting factors and terminators advance …
engineering.•New catalogue of promoters, trans-acting factors and terminators advance …
[HTML][HTML] CRISPRi screens reveal genes modulating yeast growth in lignocellulose hydrolysate
F Gutmann, C Jann, F Pereira, A Johansson… - Biotechnology for …, 2021 - Springer
Background Baker's yeast is a widely used eukaryotic cell factory, producing a diverse range
of compounds including biofuels and fine chemicals. The use of lignocellulose as feedstock …
of compounds including biofuels and fine chemicals. The use of lignocellulose as feedstock …