[HTML][HTML] Microbial applications for sustainable space exploration beyond low Earth orbit

AP Koehle, SL Brumwell, EP Seto, AM Lynch… - npj …, 2023 - nature.com
With the construction of the International Space Station, humans have been continuously
living and working in space for 22 years. Microbial studies in space and other extreme …

Evaluation of probiotic potential of autochthonous lactobacilli strains isolated from Zabuli yellow kashk, an Iranian dairy product

A Vasiee, F Falah, SA Mortazavi - Journal of Applied …, 2022 - academic.oup.com
Aims The aim of this study was to evaluate the probiotic potential and anti‐biofilm activity of
five lactobacilli strains which isolated and identified from an Iranian product. Methods and …

CRISPR-Cas systems of lactic acid bacteria and applications in food science

Y Cui, X Qu - Biotechnology Advances, 2024 - Elsevier
CRISPR-Cas (Clustered regularly interspaced short palindromic repeats-CRISPR
associated proteins) systems are widely distributed in lactic acid bacteria (LAB), contributing …

Conjugation-Based Genome Engineering in Deinococcus radiodurans

SL Brumwell, KD Van Belois, DJ Giguere… - ACS Synthetic …, 2022 - ACS Publications
Deinococcus radiodurans has become an attractive microbial platform for the study of
extremophile biology and industrial bioproduction. To improve the genomic manipulation …

[HTML][HTML] Multi-omics analysis reveals the mechanism of Lacticaseibacillus paracasei IMAUJBC1 in alleviating hyperlipidemia

F Cheng, K Zhang, X Yang, L Shi, Y Wei… - Journal of Functional …, 2024 - Elsevier
This study investigated the alleviating effect of Lacticaseibacillus paracasei IMAUJBC1 on
hyperlipidemia and explored the associated mechanism by combining microbiome …

[HTML][HTML] Enabling low-cost and robust essentiality studies with high-throughput transposon mutagenesis (HTTM)

A Champie, A De Grandmaison, S Jeanneau… - Plos one, 2023 - journals.plos.org
Transposon-insertion sequencing (TIS) methods couple high density transposon
mutagenesis with next-generation sequencing and are commonly used to identify essential …

[HTML][HTML] In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1

M Blanch-Asensio, S Dey, S Sankaran - PLOS one, 2023 - journals.plos.org
Lactobacilli are gram-positive bacteria that are growing in importance for the healthcare
industry and genetically engineering them as living therapeutics is highly sought after …

In vivo delivery of functional Cas: DNA nucleoprotein complexes into recipient bacteria through a Type IV Secretion System

DL Guzman-Herrador, A Fernandez-Gomez… - bioRxiv, 2024 - biorxiv.org
CRISPR-associated (Cas) endonucleases and their derivatives are widespread tools for the
targeted genetic modification of both prokaryotic and eukaryotic genomes. A critical step of …

Gibson Assembly-based direct cloning of plasmid DNA in Lactiplantibacillus plantarum WCSF1

M Blanch Asensio, S Dey, S Sankaran - bioRxiv, 2022 - biorxiv.org
Lactobacilli are gram-positive bacteria that are growing in importance for the healthcare
industry and genetically engineering them as living therapeutics is highly sought after …

Transformuojamų termofilinių bakterijų atranka ir jų genetinės transformacijos optimizavimas

M Maselytė - 2023 - epublications.vu.lt
Abstract [eng] Thermophilic bacteria are extremophilic microorganisms which are capable of
growing at high temperatures (> 45 &# 8451;). These bacteria are extremely important in …