Nucleoid-associated protein HU: A lilliputian in gene regulation of bacterial virulence

P Stojkova, P Spidlova, J Stulik - Frontiers in cellular and infection …, 2019 - frontiersin.org
Nucleoid-associated proteins belong to a group of small but abundant proteins in bacterial
cells. These transcription regulators are responsible for many important cellular processes …

Small prokaryotic DNA-binding proteins protect genome integrity throughout the life cycle

K Molan, D Žgur Bertok - International journal of molecular sciences, 2022 - mdpi.com
Genomes of all organisms are persistently threatened by endogenous and exogenous
assaults. Bacterial mechanisms of genome maintenance must provide protection throughout …

CcrZ is a pneumococcal spatiotemporal cell cycle regulator that interacts with FtsZ and controls DNA replication by modulating the activity of DnaA

C Gallay, S Sanselicio, ME Anderson, YM Soh… - Nature …, 2021 - nature.com
Most bacteria replicate and segregate their DNA concomitantly while growing, before cell
division takes place. How bacteria synchronize these different cell cycle events to ensure …

Three orphan histidine kinases inhibit Clostridioides difficile sporulation

AN Edwards, D Wetzel, MA DiCandia… - Journal of …, 2022 - Am Soc Microbiol
The ability of the anaerobic gastrointestinal pathogen Clostridioides difficile to survive
outside the host relies on the formation of dormant endospores. Spore formation is …

In Vivo Detection of Metabolic Fluctuations in Real Time Using the NanoBiT Technology Based on PII Signalling Protein Interactions

R Rozbeh, K Forchhammer - International Journal of Molecular Sciences, 2024 - mdpi.com
New protein-fragment complementation assays (PCA) have successfully been developed to
characterize protein–protein interactions in vitro and in vivo. Notably, the NanoBiT …

Identification of functional Spo0A residues critical for sporulation in Clostridioides difficile

MA DiCandia, AN Edwards, JB Jones… - Journal of molecular …, 2022 - Elsevier
Clostridioides difficile is an anaerobic, Gram-positive pathogen that is responsible for C.
difficile infection (CDI). To survive in the environment and spread to new hosts, C. difficile …

Plasmonic Assemblies for Real‐Time Single‐Molecule Biosensing

RE Armstrong, M Horáček, P Zijlstra - Small, 2020 - Wiley Online Library
Their tunable optical properties and versatile surface functionalization have sparked
applications of plasmonic assemblies in the fields of biosensing, nonlinear optics, and …

Studies on the PII-PipX-NtcA Regulatory Axis of Cyanobacteria Provide Novel Insights into the Advantages and Limitations of Two-Hybrid Systems for Protein …

P Salinas, S Bibak, R Cantos, L Tremiño… - International Journal of …, 2024 - mdpi.com
Yeast two-hybrid approaches, which are based on fusion proteins that must co-localise to
the nucleus to reconstitute the transcriptional activity of GAL4, have greatly contributed to our …

The (p)ppGpp-binding GTPase Era promotes rRNA processing and cold adaptation in Staphylococcus aureus

A Wood, SE Irving, DJ Bennison, RM Corrigan - PLoS Genetics, 2019 - journals.plos.org
Ribosome assembly cofactors are widely conserved across all domains of life. One such
group, the ribosome-associated GTPases (RA-GTPase), act as molecular switches to …

Potential role of the host-derived cell-wall binding domain of endolysin CD16/50L as a molecular anchor in preservation of uninfected Clostridioides difficile for new …

W Phothichaisri, S Chankhamhaengdecha… - Microbiology …, 2022 - Am Soc Microbiol
Endolysin is a phage-encoded cell-wall hydrolase which degrades the peptidoglycan layer
of the bacterial cell wall. The enzyme is often expressed at the late stage of the phage lytic …