CRISPR-Cas9 has revolutionized genome engineering by allowing precise introductions of
DNA double-strand breaks (DSBs). However, genome engineering in bacteria is still a …

The conserved cyanobacterial protein PipX is part of a complex interaction network with
regulators involved in essential processes that include metabolic homeostasis and ribosome …

To survive in the host, pathogenic bacteria need to be able to react to the unfavourable
conditions that they encounter, like low pH, elevated temperatures, antimicrobial peptides …

Faithful DNA replication is crucial for viability of cells across all kingdoms. Targeting DNA
replication is a viable strategy for inhibition of bacterial pathogens. Clostridioides difficile is …

Most bacteria replicate and segregate their DNA concomitantly while growing, before cell
division takes place. How bacteria synchronize these different cell cycle events to ensure …

Antibiotic resistance is transcriptionally controlled and activated in response to antibiotics.
Extracytoplasmic function σ factors are specialized transcription factors that are activated in …

Biopolymers such as proteins and nucleic acids participate in many biological mechanisms
such as catalysis, gene expression or DNA recombination. To execute their function …

Clostridium saccharoperbutylacetonicum is an anaerobe that is growing in importance for
biotechnological applications. In recent years, significant strides have been made in …

Clostridioides difficile is a Gram-positive, obligately anaerobic microorganism that is of
considerable medical concern as a nosocomial and community-acquired human pathogen …

Objectives Fluorescence microscopy is a valuable tool to study a broad variety of bacterial
cell components and dynamics thereof. For Clostridioides difficile, the fluorescent proteins …