Protein cages: from fundamentals to advanced applications

TGW Edwardson, MD Levasseur, S Tetter… - Chemical …, 2022 - ACS Publications
Proteins that self-assemble into polyhedral shell-like structures are useful molecular
containers both in nature and in the laboratory. Here we review efforts to repurpose diverse …

Encapsulins

TW Giessen - Annual review of biochemistry, 2022 - annualreviews.org
Subcellular compartmentalization is a defining feature of all cells. In prokaryotes,
compartmentalization is generally achieved via protein-based strategies. The two main …

Advances in the bacterial organelles for CO2 fixation

LN Liu - Trends in Microbiology, 2022 - cell.com
Carboxysomes are a family of bacterial microcompartments (BMCs), present in all
cyanobacteria and some proteobacteria, which encapsulate the primary CO 2-fixing …

α-carboxysome size is controlled by the disordered scaffold protein CsoS2

LM Oltrogge, AW Chen, T Chaijarasphong… - Biochemistry, 2023 - ACS Publications
Carboxysomes are protein microcompartments that function in the bacterial CO2
concentrating mechanism (CCM) to facilitate CO2 assimilation. To do so, carboxysomes …

Designing intracellular compartments for efficient engineered microbial cell factories

R Wang, X Liu, B Lv, W Sun, C Li - ACS Synthetic Biology, 2023 - ACS Publications
With the rapid development of synthetic biology, various kinds of microbial cell factories
(MCFs) have been successfully constructed to produce high-value-added compounds …

Inferring assembly-curving trends of bacterial micro-compartment shell hexamers from crystal structure arrangements

LF Garcia-Alles, M Fuentes-Cabrera… - PLOS Computational …, 2023 - journals.plos.org
Bacterial microcompartments (BMC) are complex macromolecular assemblies that
participate in varied chemical processes in about one fourth of bacterial species. BMC …

Nanoengineering Carboxysome Shells for Protein Cages with Programmable Cargo Targeting

T Li, P Chang, W Chen, Z Shi, C Xue, GF Dykes… - ACS …, 2024 - ACS Publications
Protein nanocages have emerged as promising candidates for enzyme immobilization and
cargo delivery in biotechnology and nanotechnology. Carboxysomes are natural …

Getting closer to decrypting the phase transitions of bacterial biomolecules

K Sołtys, A Tarczewska, D Bystranowska, N Sozańska - Biomolecules, 2022 - mdpi.com
Liquid–liquid phase separation (LLPS) of biomolecules has emerged as a new paradigm in
cell biology, and the process is one proposed mechanism for the formation of membraneless …

Formalizing Coarse-Grained Representations of Anisotropic Interactions at Multimeric Protein Interfaces Using Virtual Sites

LF Christians, EV Halingstad, E Kram… - The Journal of …, 2024 - ACS Publications
Molecular simulations of biomacromolecules that assemble into multimeric complexes
remain a challenge due to computationally inaccessible length and time scales. Low …

Assessment of oligomerization of bacterial micro-compartment shell components with the tripartite GFP reporter technology

L Barthe, V Soldan, LF Garcia-Alles - Plos one, 2023 - journals.plos.org
Bacterial micro-compartments (BMC) are complex macromolecular assemblies that
participate in varied metabolic processes in about 20% of bacterial species. Most of these …