CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Recent advances in microbial ecology and synthetic biology have the potential to mitigate
damage caused by anthropogenic activities that are deleteriously impacting Earth's soil …

To support an ever-increasing population, modern agriculture faces numerous challenges
that pose major threats to global food and energy security. Plant-associated microbes, with …

Targeted saturation mutagenesis of crop genes could be applied to produce genetic variants
with improved agronomic performance. However, tools for directed evolution of plant genes …

Abstract Machine learning provides researchers a unique opportunity to make metabolic
engineering more predictable. In this review, we offer an introduction to this discipline in …

Metabolic engineering aims to develop efficient cell factories by rewiring cellular
metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae …

Metabolic engineering allows development of microbial strains efficiently producing
chemicals and materials, but it requires much time, effort, and cost to make the strains …

The past several years have seen an explosion in development of applications for the
CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to …

Electroactive bacteria produce or consume electrical current by moving electrons to and
from extracellular acceptors and donors. This specialized process, known as extracellular …