Non-invasive classification of macrophage polarisation by 2P-FLIM and machine learning
In this study, we utilise fluorescence lifetime imaging of NAD (P) H-based cellular
autofluorescence as a non-invasive modality to classify two contrasting states of human …
autofluorescence as a non-invasive modality to classify two contrasting states of human …
Metabolic imaging using two-photon excited NADH intensity and fluorescence lifetime imaging
J Vergen, C Hecht, LV Zholudeva… - Microscopy and …, 2012 - academic.oup.com
Metabolism and mitochondrial dysfunction are known to be involved in many different
disease states. We have employed two-photon fluorescence imaging of intrinsic …
disease states. We have employed two-photon fluorescence imaging of intrinsic …
Multidimensional non-linear laser imaging of Basal Cell Carcinoma
We have used a multidimensional non-linear laser imaging approach to visualize ex-vivo
samples of basal cell carcinoma (BCC). A combination of several non-linear laser imaging …
samples of basal cell carcinoma (BCC). A combination of several non-linear laser imaging …
Fluorescence quenching of free and bound NADH in HeLa cells determined by hyperspectral imaging and unmixing of cell autofluorescence
Carbonyl cyanide-p-trifluoro methoxyphenylhydrazone (FCCP) is a well-known
mitochondrial uncoupling agent. We examined FCCP-induced fluorescence quenching of …
mitochondrial uncoupling agent. We examined FCCP-induced fluorescence quenching of …
A hyperspectral fluorescence lifetime probe for skin cancer diagnosis
PAA De Beule, C Dunsby, NP Galletly… - Review of scientific …, 2007 - pubs.aip.org
The autofluorescence of biological tissue can be exploited for the detection and diagnosis of
disease but, to date, its complex nature and relatively weak signal levels have impeded its …
disease but, to date, its complex nature and relatively weak signal levels have impeded its …
[HTML][HTML] Impact of macular pigment on fundus autofluorescence lifetimes
L Sauer, D Schweitzer, L Ramm… - … & visual science, 2015 - jov.arvojournals.org
Purpose: To characterize the macular region and to investigate the influence of the macular
pigment (MP) on fundus autofluorescence (FAF) lifetimes in vivo. Methods: Forty-eight …
pigment (MP) on fundus autofluorescence (FAF) lifetimes in vivo. Methods: Forty-eight …
Resolution of 4 components in the same pixel in FLIM images using the phasor approach
In several cellular systems, the phasor FLIM approach has shown the existence of more than
2 components in the same pixel, a typical example being free and bound NADH. In order to …
2 components in the same pixel, a typical example being free and bound NADH. In order to …
Intravital microscopy for real-time monitoring of drug delivery and nanobiological processes
Intravital microscopy (IVM) expands our understanding of cellular and molecular processes,
with applications ranging from fundamental biology to (patho) physiology and immunology …
with applications ranging from fundamental biology to (patho) physiology and immunology …
Multicomponent analysis of phasor plot in a single pixel to calculate changes of metabolic trajectory in biological systems
Phasor FLIM in cells undergoing oxidative stress and in mice liver sections have shown the
presence of a third autofluorescent component indicative of lipid droplets along with free and …
presence of a third autofluorescent component indicative of lipid droplets along with free and …
[HTML][HTML] Advances and challenges in label-free nonlinear optical imaging using two-photon excitation fluorescence and second harmonic generation for cancer …
G Thomas, J van Voskuilen, HC Gerritsen… - … of Photochemistry and …, 2014 - Elsevier
Nonlinear optical imaging (NLOI) has emerged to be a promising tool for bio-medical
imaging in recent times. Among the various applications of NLOI, its utility is the most …
imaging in recent times. Among the various applications of NLOI, its utility is the most …