The extent to which nanoscale‐engineered systems cross intact human skin and can exert
pharmacological effects in viable epidermis is controversial. This research seeks to develop …

Photon counting detectors currently used in fluorescence lifetime microscopy have a number
of deficiencies that result in less‐than‐ideal signal‐to‐noise ratio of the lifetimes obtained …

Two-photon fluorescence lifetime imaging microscopy (FLIM) is widely used to capture
autofluorescence signals from cellular components to investigate dynamic physiological …

Hyperspectral imaging uses spectral and spatial image information for target detection and
classification. In this work hyperspectral autofluorescence imaging was applied to patient …

In live tissue, alterations in metabolism induce changes in the fluorescence decay of the
biological coenzyme NAD (P) H, the mechanism of which is not well understood. In this …

Two-photon fluorescence lifetime microscopy (2P-FLIM) is a non-invasive optical technique
that can obtain cellular metabolism information based on the intrinsic autofluorescence …

Measurement of endogenous free and bound NAD (P) H relative concentrations in living
cells is a useful method for monitoring aspects of cellular metabolism, because the …

Bacterial populations exhibit a range of metabolic states influenced by their environment,
intra-and interspecies interactions. The identification of bacterial metabolic states and …

There is a growing body of literature showing the usefulness of multiphoton tomography
(MPT) and fluorescence lifetime imaging for in situ characterization of skin constituents and …

Ex vivo human skin has been used extensively for cosmeceutical and drug delivery studies,
transplantable skin allografts, or skin flaps. However, it has a half-life of a few days due to …