All known eukaryotic and some viral mRNA capping enzymes (CEs) transfer a GMP moiety
of GTP to the 5′-diphosphate end of the acceptor RNA via a covalent enzyme-GMP …

Colorectal cancers (CRCs) are prevalent worldwide, yet current treatments remain
inadequate. Using chemical genetic screens, we identify that co-inhibition of topoisomerase …

Enzymatic processing of DNA underlies all DNA repair, yet inappropriate DNA processing
must be avoided. In vertebrates, double-strand breaks are repaired predominantly by non …

Double strand breaks pose unique problems for DNA repair, especially when broken ends
possess complex structures that interfere with standard DNA transactions. Nonhomologous …

Abstract Tyrosyl–DNA Phosphodiesterases 1 (TDP1) and 2 (TDP2) are eukaryotic enzymes
that clean-up after aberrant topoisomerase activity. While TDP1 hydrolyzes phosphotyrosyl …

Abstract Poly (ADP-Ribose)(PAR) polymerase (PARP) inhibitors represent a promising class
of novel anticancer agents. The present study explores the molecular rationale for …

Thousands of cellular single-strand breaks (SSBs) arise in cells each day, from attack of
deoxyribose and DNA bases by reactive oxygen species and other electrophilic molecules …

A number of proteins form covalent bonds with DNA as obligatory transient intermediates in
normal nuclear transactions. Drugs that trap these complexes have proven to be potent …

Topoisomerase I (TOP1) inhibitors applied in cancer therapy such as topotecan and
irinotecan are derivatives of the natural alkaloid camptothecin (CPT). The mechanism of …

Human tyrosyl–DNA phosphodiesterase (TDP1) hydrolyzes the phosphodiester bond at a
DNA 3′ end linked to a tyrosyl moiety. This type of linkage is found at stalled …