NF-κB activates the primary inflammatory response pathway responsible for methicillin-
resistant Staphylococcus aureus (MRSA)-induced lung inflammation and injury. Here, we …

With the availability of the whole genome sequence of Escherichia coli or Corynebacterium
glutamicum, strategies for directed DNA manipulation have developed rapidly. DNA …

Transcription elongation has been recognized as a rate-limiting step for the expression of
signal-inducible genes. Through recruitment of positive transcription elongation factor P …

Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is a typical single-layer capsid
dsRNA virus belonging to the genus Cypovirus in the family Reoviridae. The results of cryo …

Site-directed mutagenesis (SDM) is a powerful tool to create defined collections of protein
variants for experimental and clinical purposes, but effectiveness is compromised when a …

A rapid and efficient site-directed mutagenesis (SDM) protocol based on two separate
polymerase chain reaction (PCR) amplifications and homologous recombination in …

Primer design is essential to conduct whole plasmid site‐directed mutagenesis for protein
study. Traditionally, primers of mutagenesis are designed manually that is time‐consuming …

Site-directed mutagenesis (SDM) methods are very important in modern molecular biology,
biochemistry, and protein engineering. Here, we present a novel SDM method that can be …

Background Distinguishing desired mutants from parental templates and undesired mutants
is a problem not well solved in Quikchange™ mutagenesis. Although Dpn I digestion can …

Cytokinesis involves temporally and spatially coordinated action of the cell cycle,
cytoskeletal and membrane systems to achieve separation of daughter cells. The septation …