Conventional and molecular techniques from simple breeding to speed breeding in crop plants: recent advances and future outlook
In most crop breeding programs, the rate of yield increment is insufficient to cope with the
increased food demand caused by a rapidly expanding global population. In plant breeding …
increased food demand caused by a rapidly expanding global population. In plant breeding …
CRISPR/Cas genome editing and precision plant breeding in agriculture
K Chen, Y Wang, R Zhang, H Zhang… - Annual review of plant …, 2019 - annualreviews.org
Enhanced agricultural production through innovative breeding technology is urgently
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …
The emerging and uncultivated potential of CRISPR technology in plant science
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
Applications and potential of genome editing in crop improvement
Genome-editing tools provide advanced biotechnological techniques that enable the
precise and efficient targeted modification of an organism's genome. Genome-editing …
precise and efficient targeted modification of an organism's genome. Genome-editing …
CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement
Since it was first recognized in bacteria and archaea as a mechanism for innate viral
immunity in the early 2010s, clustered regularly interspaced short palindromic repeats …
immunity in the early 2010s, clustered regularly interspaced short palindromic repeats …
[HTML][HTML] Construct design for CRISPR/Cas-based genome editing in plants
CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
Y Zhang, Q Ren, X Tang, S Liu, AA Malzahn… - Nature …, 2021 - nature.com
CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …
Engineering salinity tolerance in plants: progress and prospects
Main conclusion There is a need to integrate conceptual framework based on the current
understanding of salt stress responses with different approaches for manipulating and …
understanding of salt stress responses with different approaches for manipulating and …
Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …
Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis
AA Malzahn, X Tang, K Lee, Q Ren, S Sretenovic… - BMC biology, 2019 - Springer
Background CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct
features that have expanded genome editing capabilities. Cas12a-mediated genome editing …
features that have expanded genome editing capabilities. Cas12a-mediated genome editing …