Chemistry of lipid nanoparticles for RNA delivery
Conspectus Lipid nanoparticles (LNPs) are a type of lipid vesicles that possess a
homogeneous lipid core. These vesicles are widely used in small-molecule drug and …
homogeneous lipid core. These vesicles are widely used in small-molecule drug and …
Self-assembled mRNA vaccines
Abstract mRNA vaccines have evolved from being a mere curiosity to emerging as COVID-
19 vaccine front-runners. Recent advancements in the field of RNA technology, vaccinology …
19 vaccine front-runners. Recent advancements in the field of RNA technology, vaccinology …
Viral membrane fusion: a dance between proteins and lipids
There are at least 21 families of enveloped viruses that infect mammals, and many contain
members of high concern for global human health. All enveloped viruses have a dedicated …
members of high concern for global human health. All enveloped viruses have a dedicated …
Membrane attachment and fusion of HIV-1, influenza A, and SARS-CoV-2: resolving the mechanisms with biophysical methods
Attachment to and fusion with cell membranes are two major steps in the replication cycle of
many human viruses. We focus on these steps for three enveloped viruses, ie, HIV-1, IAVs …
many human viruses. We focus on these steps for three enveloped viruses, ie, HIV-1, IAVs …
The ACE2 receptor accelerates but is not biochemically required for SARS-CoV-2 membrane fusion
The SARS-CoV-2 coronavirus infects human cells via the ACE2 receptor. Structural
evidence suggests that ACE2 may not just serve as an attachment factor but also …
evidence suggests that ACE2 may not just serve as an attachment factor but also …
[PDF][PDF] Measuring single-virus fusion kinetics using an assay for nucleic acid exposure
AMV Giraldo, S Mannsverk, PM Kasson - Biophysical Journal, 2022 - cell.com
The kinetics by which individual enveloped viruses fuse with membranes provide an
important window into viral-entry mechanisms. We have developed a real-time assay using …
important window into viral-entry mechanisms. We have developed a real-time assay using …
Precise triggering and chemical control of single-virus fusion within endosomes
Many enveloped viruses infect cells within endocytic compartments. The pH drop that
accompanies endosomal maturation, often in conjunction with proteolysis, triggers viral …
accompanies endosomal maturation, often in conjunction with proteolysis, triggers viral …
Single-virus fusion measurements reveal multiple mechanistically equivalent pathways for SARS-CoV-2 entry
A Sengar, M Cervantes, ST Bondalapati… - Journal of …, 2023 - Am Soc Microbiol
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) binds to cell surface
receptors and is activated for membrane fusion and cell entry via proteolytic cleavage …
receptors and is activated for membrane fusion and cell entry via proteolytic cleavage …
[PDF][PDF] Single-virus assay reveals membrane determinants and mechanistic features of Sendai virus binding
A Lam, OO Kirkland, PF Anderson, N Seetharaman… - Biophysical …, 2022 - cell.com
Sendai virus (SeV, formally murine respirovirus) is a membrane-enveloped, negative-sense
RNA virus in the Paramyxoviridae family and is closely related to human parainfluenza …
RNA virus in the Paramyxoviridae family and is closely related to human parainfluenza …
Recent developments in single-virus fusion assay
S Haldar - The Journal of Membrane Biology, 2022 - Springer
Viral infection is a global health hazard. A crucial step in the infection cycle of enveloped
viruses is the fusion of viral and host cellular membranes, which permits the transfer of the …
viruses is the fusion of viral and host cellular membranes, which permits the transfer of the …