Quantitative phase imaging for label-free analysis of cancer cells—focus on digital holographic microscopy

Z El-Schich, A Leida Mölder, A Gjörloff Wingren - Applied Sciences, 2018 - mdpi.com
To understand complex biological processes, scientists must gain insight into the function of
individual living cells. In contrast to the imaging of fixed cells, where a single snapshot of the …

Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion

O Tolde, A Gandalovičová, A Křížová, P Veselý… - Scientific reports, 2018 - nature.com
Observation and analysis of cancer cell behaviour in 3D environment is essential for full
understanding of the mechanisms of cancer cell invasion. However, label-free imaging of …

Geometric-phase microscopy for quantitative phase imaging of isotropic, birefringent and space-variant polarization samples

P Bouchal, L Štrbková, Z Dostál, R Chmelík… - Scientific reports, 2019 - nature.com
We present geometric-phase microscopy allowing a multipurpose quantitative phase
imaging in which the ground-truth phase is restored by quantifying the phase retardance …

Quantitative phase imaging of spreading fibroblasts identifies the role of focal adhesion kinase in the stabilization of the cell rear

O Ramaniuk, Z Klímová, T Groušl, T Vomastek - Biomolecules, 2020 - mdpi.com
Cells attaching to the extracellular matrix spontaneously acquire front–rear polarity. This self-
organization process comprises spatial activation of polarity signaling networks and the …

Comparison of holotomographic microscopy and coherence‐controlled holographic microscopy

V Chvalova, T Vomastek, T Grousl - Journal of Microscopy, 2024 - Wiley Online Library
Quantitative phase imaging (QPI) is a powerful tool for label‐free visualisation of living cells.
Here, we compare two QPI microscopes–the Telight Q‐Phase microscope and the Nanolive …

Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic …

M Feith, Y Zhang, JL Persson, J Balvan, Z El-Schich… - Photonics, 2022 - mdpi.com
Colorectal cancer (CRC) is the second most metastatic disease with the majority of cases
detected in Western countries. Metastases are formed by circulating altered phenotype …

In focus in HCB

DJ Taatjes, J Roth - Histochemistry and cell biology, 2022 - Springer
This last Editorial for 2022 begins with presentation of the “Most popular articles published in
HCB in 2020.” We then follow with highlights from two Original Articles investigating the …

[PDF][PDF] Detekce a analýza nádorových buněk pomocí fluorescenčních molekulárně imprintovaných polymerů a 3D holografícké mikroskopie

M Feith - 2022 - is.muni.cz
Nádorové buňky vykazují výrazné změny v glykosylaci buněčného povrchu v důsledku
dysregulovaných glykosyltransferáz a glykosidáz. Zvýšení exprese sialylovaných glykanů je …

Quantitative phase imaging during cell division based on dual-channel microscopic interferometry system

S Liu, D Zheng, J Li, J Tian, L Zhong… - IEEE Photonics …, 2019 - ieeexplore.ieee.org
Quantitative phase imaging (QPI) during cell division is implemented by ourhomemade dual-
channel microscopic interferometry (DCMI) system. A pair of interferograms with a fixed …

Phase Retrieval Method Based on Transport of Intensity Equation with Microscope Single Field of View

H Cheng, R Wang, F Zhang, W Bao… - Sensor Networks and …, 2021 - Springer
In microscopic imaging, transport of intensity equation (TIE) is an effective phase retrieval
method. In order to effectively calculate the lost phase from the intensity information, a phase …