Modular cloning in plant cells

M Karimi, B De Meyer, P Hilson - Trends in plant science, 2005 - cell.com
New plant genes are being discovered at a rapid pace. Yet, in most cases, their precise
function remains elusive. The recent advent of recombinational cloning techniques has …

Recombinational cloning with plant gateway vectors

M Karimi, A Depicker, P Hilson - Plant physiology, 2007 - academic.oup.com
The study of biological systems relies to a large extent on DNA cloning technologies
enabling the analysis of recombinant genes through transgenic research. In this context, the …

[HTML][HTML] Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks

L Yang, F Machin, S Wang, E Saplaoura… - Nature …, 2023 - nature.com
Generation of stable gene-edited plant lines using clustered regularly interspaced short
palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy …

Plant “helper” immune receptors are Ca2+-permeable nonselective cation channels

P Jacob, NH Kim, F Wu, F El-Kasmi, Y Chi, WG Walton… - Science, 2021 - science.org
Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell
death. In Arabidopsis, a subfamily of “helper” NLRs is required by many “sensor” NLRs …

[HTML][HTML] GABA signalling modulates stomatal opening to enhance plant water use efficiency and drought resilience

B Xu, Y Long, X Feng, X Zhu, N Sai, L Chirkova… - Nature …, 2021 - nature.com
The non-protein amino acid γ-aminobutyric acid (GABA) has been proposed to be an
ancient messenger for cellular communication conserved across biological kingdoms. GABA …

A multipurpose toolkit to enable advanced genome engineering in plants

T Čermák, SJ Curtin, J Gil-Humanes, R Čegan… - The Plant …, 2017 - academic.oup.com
We report a comprehensive toolkit that enables targeted, specific modification of monocot
and dicot genomes using a variety of genome engineering approaches. Our reagents …

A CRISPR–Cpf1 system for efficient genome editing and transcriptional repression in plants

X Tang, LG Lowder, T Zhang, AA Malzahn, X Zheng… - Nature plants, 2017 - nature.com
Clustered regularly interspaced short palindromic repeats (CRISPR)–Cpf1 has emerged as
an effective genome editing tool in animals. Here we compare the activity of Cpf1 from …

[HTML][HTML] A CRISPR/Cas9 toolkit for multiplex genome editing in plants

HL Xing, L Dong, ZP Wang, HY Zhang, CY Han, B Liu… - BMC plant biology, 2014 - Springer
Background To accelerate the application of the CRISPR/Cas9 (clustered regularly
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …

A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation

LG Lowder, D Zhang, NJ Baltes, JW Paul III… - Plant …, 2015 - academic.oup.com
The relative ease, speed, and biological scope of clustered regularly interspaced short
palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for …

[HTML][HTML] ATG8-binding UIM proteins define a new class of autophagy adaptors and receptors

RS Marshall, Z Hua, S Mali, F McLoughlin, RD Vierstra - Cell, 2019 - cell.com
During autophagy, vesicle dynamics and cargo recruitment are driven by numerous
adaptors and receptors that become tethered to the phagophore through interactions with …