Genetic integrity of cryopreserved plant cells: a review

K Harding - CryoLetters, 2004 - ingentaconnect.com
Cryopreservation techniques exist for some 100 plant species. Cryopreservation for the long-
term conservation of in vitro germplasm results in the exposure of tissues to physical …

[HTML][HTML] Critical role of regrowth conditions in post-cryopreservation of in vitro plant germplasm

E Popova, I Kulichenko, HH Kim - Biology, 2023 - mdpi.com
Simple Summary Optimization of regrowth conditions is critical for the successful
cryopreservation of in vitro plant germplasm. This review discusses the five major strategies …

Genetic fidelity of cryopreserved embryogenic cultures of open-pollinated Abies cephalonica

TS Aronen, J Krajnakova, HM Häggman, LA Ryynänen - Plant Science, 1999 - Elsevier
The aim of the present study was to apply cryopreservation for the embryogenic cultures of
open-pollinated Abies cephalonica, as well as to test the genetic fidelity of the cryopreserved …

Scots Pine (Pinus sylvestris L.)

UK Krakau, M Liesebach, T Aronen… - Forest tree breeding in …, 2013 - Springer
Scots pine (Pinus sylvestris L.) is an economically very important and scientifically well
investigated tree species of the northern hemisphere occupying large areas with different …

Potential applications of cryogenic technologies to plant genetic improvement and pathogen eradication

B Wang, RR Wang, ZH Cui, WL Bi, JW Li, BQ Li… - Biotechnology …, 2014 - Elsevier
Rapid increases in human populations provide a great challenge to ensure that adequate
quantities of food are available. Sustainable development of agricultural production by …

In vitro encapsulation based short term storage and assessment of genetic homogeneity in regenerated Ansellia africana (Leopard orchid) using gene targeted …

P Bhattacharyya, V Kumar, J Van Staden - Plant Cell, Tissue and Organ …, 2018 - Springer
An efficient short term storage protocol was developed for Ansellia africana, a vulnerable
medicinal orchid of Africa using encapsulated protocorm-like bodies (PLBs) induced from …

Clonal plant production from self- and cross-pollinated seed families of Pinus sylvestris (L.) through somatic embryogenesis

MA Lelu-Walter, M Bernier-Cardou… - Plant Cell, Tissue and …, 2008 - Springer
Several factors affecting somatic embryogenesis (SE) in Pinus sylvestris from self-and cross-
pollinated seed families were studied with the aim of producing large quantities of clonal …

Cryopreservation of human bone marrow‐derived mesenchymal stem cells with reduced dimethylsulfoxide and well‐defined freezing solutions

Y Liu, X Xu, X Ma, E Martin‐Rendon… - Biotechnology …, 2010 - Wiley Online Library
The aim of this study is to investigate the feasibility of using well defined, serum‐free
freezing solutions with a reduced level of dimethylsulfoxide (DMSO) of 7.5, 5, and 2.5%(v/v) …

Genetic stability of cryopreserved shoot tips of Rubus germplasm

NRF Castillo, NV Bassil, S Wada, BM Reed - In Vitro Cellular & …, 2010 - Springer
Questions often arise concerning the genetic stability of plant materials stored in liquid
nitrogen for long time periods. This study examined the genetic stability of cryopreserved …

Assessment of genetic and epigenetic changes following cryopreservation in papaya

A Kaity, SE Ashmore, RA Drew, ME Dulloo - Plant Cell Reports, 2008 - Springer
A vitrification based cryopreservation technique for storage of in vitro shoot tips of papaya
has been tested to ensure applicability across a range of genotypes and to assess the …