Parameters to identify good quality oocytes and embryos in cattle

C Wrenzycki - Reproduction, Fertility and Development, 2021 - CSIRO Publishing
Oocyte/embryo selection methodologies are either invasive or noninvasive and can be
applied at various stages of development from the oocyte to cleaved embryos and up to the …

Current status of the intrafollicular transfer of immature oocytes (IFIOT) in cattle: a review

ACCV Nicolás, MAN Dode - Animal Reproduction Science, 2024 - Elsevier
ABSTRACT Intrafollicular Transfer of Immature Oocytes (IFIOT) has emerged as an
alternative to the currently used systems for bovine embryo production. This technique …

Cleavage kinetics is a better indicator of embryonic developmental competency than brilliant cresyl blue staining of oocytes

J Nix, MA Marrella, MA Oliver, M Rhoads… - Animal Reproduction …, 2023 - Elsevier
In vitro production of embryos (IVP) is a valuable technology to produce embryos of high
genetic value. Despite advances in IVP, the efficiency of culture systems remains low. One …

Optical coherence microscopy allows for quality assessment of immature mouse oocytes

M Fluks, S Tamborski, M Szkulmowski… - Reproduction, 2022 - rep.bioscientifica.com
In brief Optical coherence microscopy is a label-free and non-invasive imaging technique
capable of 3D subcellular structure visualization. Here we show that this method allows for …

Simple and efficient chemically defined in vitro maturation and embryo culture system for bovine embryos

ME Arias, T Vargas, V Gallardo, L Aguila, R Felmer - Animals, 2022 - mdpi.com
Simple Summary Supplementation of the culture media for the in vitro production of bovine
embryos with fetal bovine serum (FBS) is associated with inconsistent outcomes. This study …

Improving Cryopreservation Efficiency and Pregnancy Rate through Superovulation with Follicle-Stimulating Hormone in Korean Hanwoo Cows via Ovum Pick Up

D Kim, J Yi - Veterinary Sciences, 2023 - mdpi.com
Simple Summary Ovum pick up (OPU) methods play a significant role in oocyte collection
worldwide and are the most efficient means of enhancing genetic advancement through …

Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality

A Gad, M Murin, A Bartkova, V Kinterova… - Journal of Animal …, 2022 - Springer
Abstract Background Ovarian follicular fluids (FFs) contain several kinds of regulatory factors
that maintain a suitable microenvironment for oocyte development. Extracellular vesicles …

Brilliant Cresyl Blue Negative Oocytes Show a Reduced Competence for Embryo Development after In Vitro Fertilisation with Sperm Exposed to Oxidative Stress

L Bittner-Schwerda, C Herrera, S Wyck, E Malama… - Animals, 2023 - mdpi.com
Simple Summary Oxidatively stressed sperm can fertilise oocytes and, hence, transfer its
damaged genome to a progeny. Sperm cannot repair its own genome, therefore the oocyte's …

In vitro embryos of Romosinuano and Tropical Milking cattle during three seasons in Veracruz, Mexico

F Rosales-Martínez, CM Becerril-Pérez… - Animals, 2024 - mdpi.com
Simple Summary Heat stress in bovine females from hot tropical regions is one of the main
factors that negatively affects the success of ovum pick-up and in vitro fertilization programs …

Predicting nuclear maturation speed of oocytes from Japanese Black beef heifers through non-invasive observations during IVM: An attempt using machine learning …

TCT Ho, N Kawate, K Koyama - Theriogenology, 2023 - Elsevier
Nuclear maturation is an essential process in which oocytes acquire the competence to
develop further. However, the time required for nuclear maturation during IVM varies among …