Cultivation‐dependant assessment, diversity, and ecology of haloalkaliphilic bacteria in arid saline systems of southern Tunisia
D El Hidri, A Guesmi, A Najjari, H Cherif… - BioMed research …, 2013 - Wiley Online Library
Haloalkaliphiles are polyextremophiles adapted to grow at high salt concentrations and
alkaline pH values. In this work, we isolated 122 haloalkaliphilic bacteria upon enrichments …
alkaline pH values. In this work, we isolated 122 haloalkaliphilic bacteria upon enrichments …
Cloning and characterization of a novel intracellular serine protease (IspK) from Bacillus megaterium with a potential additive for detergents
YJ Jeong, SC Baek, H Kim - International journal of biological …, 2018 - Elsevier
A new intracellular serine protease gene of Bacillus megaterium, ispK, encoding a protein
composed of 332 amino acid residues with a predicted pI of 4.7 was cloned into Escherichia …
composed of 332 amino acid residues with a predicted pI of 4.7 was cloned into Escherichia …
Purification and characterization of novel organic-solvent-tolerant β-amylase and serine protease from a newly isolated Salimicrobium halophilum strain LY20
X Li, HY Yu - FEMS microbiology letters, 2012 - academic.oup.com
A halophilic isolate Salimicrobium halophilum strain LY20 producing extracellular amylase
and protease was isolated from Yuncheng, China. Production of both enzymes was …
and protease was isolated from Yuncheng, China. Production of both enzymes was …
Production and optimization of a commercially viable alkaline protease from a haloalkaliphilic bacterium
RH Joshi, MS Dodia, SP Singh - Biotechnology and Bioprocess …, 2008 - Springer
Twenty five haloalkaliphilic bacterial strains were isolated from sea water along the Coastal
Gujarat (India) and screened for their ability to secret alkaline proteases. Among them, a …
Gujarat (India) and screened for their ability to secret alkaline proteases. Among them, a …
Assessment of various methods for extraction of metagenomic DNA from saline habitats of coastal Gujarat (India) to explore molecular diversity
MK Purohit, SP Singh - Letters in applied microbiology, 2009 - academic.oup.com
Aims: To develop total DNA extraction protocol from saline soil for further metagenomic
applications. Methods and Results: The protocols combine the application of mechanical …
applications. Methods and Results: The protocols combine the application of mechanical …
Comparative studies on the extraction of metagenomic DNA from the saline habitats of Coastal Gujarat and Sambhar Lake, Rajasthan (India) in prospect of molecular …
PK Siddhapura, S Vanparia, MK Purohit… - International journal of …, 2010 - Elsevier
Extraction of total DNA from a given habitat assumes significance in metagenomics, due to
the requirement of inhibitor free and high quality metagenome in good quantity for …
the requirement of inhibitor free and high quality metagenome in good quantity for …
Organic solvent tolerance of an α-amylase from haloalkaliphilic bacteria as a function of pH, temperature, and salt concentrations
S Pandey, SP Singh - Applied biochemistry and biotechnology, 2012 - Springer
A haloalkaliphilic bacterium was isolated from salt-enriched soil of Mithapur, Gujarat (India)
and identified as Bacillus agaradhaerens Mi-10-6 2 based on 16S rRNA sequence analysis …
and identified as Bacillus agaradhaerens Mi-10-6 2 based on 16S rRNA sequence analysis …
Purification and characterization of a solvent‐stable protease from Geomicrobium sp. EMB2
A moderately haloalkaliphilic bacterium, Geomicrobium sp. EMB2, was isolated from the
Sambhar Salt Lake located in the western part of India. It secreted an alkaline protease …
Sambhar Salt Lake located in the western part of India. It secreted an alkaline protease …
Comparative analysis of the catalysis and stability of the native, recombinant and metagenomic alkaline proteases in organic solvents
MK Purohit, DS Rathore, G Koladiya, S Pandey… - … Science and Pollution …, 2022 - Springer
The effect of organic solvents on alkaline proteases was assessed for native, recombinant,
and metagenomically derived alkaline proteases. Their stability and the effects of …
and metagenomically derived alkaline proteases. Their stability and the effects of …
A metagenomic alkaline protease from saline habitat: cloning, over-expression and functional attributes
MK Purohit, SP Singh - International journal of biological macromolecules, 2013 - Elsevier
Metagenomics has opened new horizon to unlock the biotechnological potential for novel
enzymes. An alkaline protease gene was obtained from the total environmental DNA …
enzymes. An alkaline protease gene was obtained from the total environmental DNA …