Zona pellucida removal by acid Tyrode's solution affects pre-and post-implantation development and gene expression in mouse embryos
W Fan, T Huang, T Wu, H Bai, M Kawahara… - Biology of …, 2022 - academic.oup.com
The zona pellucida plays a crucial role in the process of fertilization to early embryonic
development, including cellular arrangement and communication between blastomeres …
development, including cellular arrangement and communication between blastomeres …
miR-17-5p in bovine oviductal fluid affects embryo development
S Aoki, Y Inoue, A Shinozawa, K Tanaka… - Molecular and Cellular …, 2022 - Elsevier
This study identified microRNAs (miRNAs) in bovine oviductal fluids (OFs) and examined the
effect of miR-17-5p in OFs on embryonic development to the blastocyst stage. Small RNA …
effect of miR-17-5p in OFs on embryonic development to the blastocyst stage. Small RNA …
miRNAs in Follicular and Oviductal Fluids Support Global DNA Demethylation in Early-Stage Embryos
S Aoki, Y Inoue, M Hamazaki, S Hara… - International Journal of …, 2024 - mdpi.com
Global methylation levels differ in in vitro-and in vivo-developed embryos. Follicular fluid
(FF) contains extracellular vesicles (EVs) containing miRNAs that affect embryonic …
(FF) contains extracellular vesicles (EVs) containing miRNAs that affect embryonic …
microRNAs associated with the quality of follicular fluids affect oocyte and early embryonic development
S Aoki, Y Inoue, S Hara, J Itou… - … Medicine and Biology, 2024 - Wiley Online Library
Purpose Oocyte and embryo quality differs significantly among individuals. Follicular fluid
(FF) is a solo environment of oocyte maturation and may flux into the oviduct …
(FF) is a solo environment of oocyte maturation and may flux into the oviduct …
Gene editing in porcine embryos using a combination of electroporation and transfection methods
K Takebayashi, M Wittayarat, Q Lin… - … in Domestic Animals, 2022 - Wiley Online Library
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats‐CRISPR‐
associated protein 9) technology is growing rapidly and has been greatly influencing the …
associated protein 9) technology is growing rapidly and has been greatly influencing the …
Embryonic MTHFR contributes to blastocyst development
H Ishitani, S Ikeda, K Egashira, M Sugimoto… - Journal of Assisted …, 2020 - Springer
Purpose Reduction in methylenetetrahydrofolate reductase (MTHFR) activity due to genetic
variations in the MTHFR gene has been controversially implicated in subfertility in human in …
variations in the MTHFR gene has been controversially implicated in subfertility in human in …
Back to the future: optimised microwell culture of individual human preimplantation stage embryos
Although in vitro culture of human embryos is a crucial step in assisted reproduction, the lack
of focused research hampers worldwide standardisation and consistent outcomes. Only …
of focused research hampers worldwide standardisation and consistent outcomes. Only …
[图书][B] Biochemistry and molecular biology compendium
RL Lundblad - 2007 - taylorfrancis.com
While biomedical investigation has greatly advanced, investigators have lost touch with and
inadvertently corrupted significant nomenclature at the foundation of their science …
inadvertently corrupted significant nomenclature at the foundation of their science …
The use of a two-step removal protocol and optimized culture conditions improve development and quality of zona free mouse embryos
W Fan, M Homma, R Xu, H Kunii, H Bai… - Biochemical and …, 2021 - Elsevier
The zona pellucida (ZP) plays an important role in both the fertilization and embryonic
development. For the successful handling of early stage blastomeres for differentiation …
development. For the successful handling of early stage blastomeres for differentiation …
The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts
F Tanihara, M Hirata, S Morikawa… - … of Reproduction and …, 2019 - jstage.jst.go.jp
The introduction of exogenous molecules into embryos is required for analyses of molecular
dynamics and specific gene functions during early embryonic development. Electroporation …
dynamics and specific gene functions during early embryonic development. Electroporation …