CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane

GS Prado, DC Rocha, LN Santos… - Frontiers in Plant …, 2024 - frontiersin.org
Gene editing technologies have opened up the possibility of manipulating the genome of
any organism in a predicted way. CRISPR technology is the most used genome editing tool …

Tending genome editing via CRISPR/Cas9-induced mutagenesis: Opportunity and challenges for yield, quality and nutritional improvement of fruit crops

A Dongariyal, AK Chandra, A Dongriyal, A Kumar… - Scientia …, 2023 - Elsevier
Fruit crops, including bananas, apples, citrus fruit, grape, berries, etc. not only offer essential
nutrients for sustaining healthy human life but also contribute to a major part of the GDP of …

Influence of Anthocyanin Expression on the Performance of Photosynthesis in Sweet Orange, Citrus sinensis (L.) Osbeck

A Cheaib, LM Mahmoud, C Vincent, N Killiny, M Dutt - Plants, 2023 - mdpi.com
Anthocyanins are a class of natural pigments that accumulate transiently or permanently in
plant tissues, often in response to abiotic and biotic stresses. They play a photoprotective …

Overexpression of the Arabidopsis NPR1 gene confers enhanced salt tolerance by regulating antioxidant and starch accumulation in citrus

LM Mahmoud, D Stanton, BH Amin, JW Grosser… - Plant Cell, Tissue and …, 2022 - Springer
Abstract The Non-Expresser of Pathogenesis-Related Genes 1 (NPR1) is a principal
regulator of plant responses to biotic and abiotic stresses. In this study, the response of …

Generation of transgene-free canker-resistant Citrus sinensis cv. Hamlin in the T0 generation through Cas12a/CBE co-editing

H Jia, AA Omar, J Xu, J Dalmendray, Y Wang… - Frontiers in Plant …, 2024 - frontiersin.org
Citrus canker disease affects citrus production. This disease is caused by Xanthomonas citri
subsp. citri (Xcc). Previous studies confirmed that during Xcc infection, PthA4, a …

Genome editing of a recalcitrant wine grape genotype by lipofectamine‐mediated delivery of CRISPR/Cas9 ribonucleoproteins to protoplasts

G Gambino, F Nuzzo, A Moine, W Chitarra… - The Plant …, 2024 - Wiley Online Library
The main bottleneck in the application of biotechnological breeding methods to woody
species is due to the in vitro regeneration recalcitrance shown by several genotypes. On the …

Cas12a RNP-mediated co-transformation enables transgene-free multiplex genome editing, long deletions, and inversions in citrus chromosome

H Su, Y Wang, J Xu, AA Omar, JW Grosser… - Frontiers in Plant …, 2024 - frontiersin.org
Introduction Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is a devastating
disease worldwide. Previously, we successfully generated canker-resistant Citrus sinensis …

[HTML][HTML] Gene editing by SSB/CRISPR-Cas9 ribonucleoprotein in bacteria

R Chai, W Sun, Z Xu, X Yao, S Chen, H Wang… - International Journal of …, 2024 - Elsevier
The application of CRISPR-Cas9 ribonucleoprotein (RNP) for gene editing is commonly
used in plants and animals, but its application in bacteria has not been reported. In this …

Creating Climate-Resilient Crops by Increasing Drought, Heat, and Salt Tolerance

T Sugumar, G Shen, J Smith, H Zhang - Plants, 2024 - mdpi.com
Over the years, the changes in the agriculture industry have been inevitable, considering the
need to feed the growing population. As the world population continues to grow, food …

Recent Advances in Tomato Gene Editing

E Larriba, O Yaroshko, JM Pérez-Pérez - International Journal of …, 2024 - mdpi.com
The use of gene-editing tools, such as zinc finger nucleases, TALEN, and CRISPR/Cas,
allows for the modification of physiological, morphological, and other characteristics in a …