Current trends in protein engineering: updates and progress
R Sinha, P Shukla - Current Protein and Peptide Science, 2019 - ingentaconnect.com
Proteins are one of the most important and resourceful biomolecules that find applications in
health, industry, medicine, research, and biotechnology. Given its tremendous relevance …
health, industry, medicine, research, and biotechnology. Given its tremendous relevance …
[HTML][HTML] Biocatalyzed synthesis of statins: A sustainable strategy for the preparation of valuable drugs
Statins, inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, are the
largest selling class of drugs prescribed for the pharmacological treatment of …
largest selling class of drugs prescribed for the pharmacological treatment of …
Semirational engineering of an aldo–keto reductase KmAKR for overcoming trade‐offs between catalytic activity and thermostability
SF Li, JY Xie, S Qiu, SY Xu, F Cheng… - Biotechnology and …, 2021 - Wiley Online Library
Enzyme engineering usually generates trade‐offs between activity, stability, and selectivity.
Herein, we report semirational engineering of an aldo–keto reductase (AKR) KmAKR for …
Herein, we report semirational engineering of an aldo–keto reductase (AKR) KmAKR for …
Biosynthesis of tert-butyl (3R, 5S)-6-chloro-3, 5-dihydroxyhexanoate by carbonyl reductase from Rhodosporidium toruloides in mono and biphasic media
ZQ Liu, L Wu, L Zheng, WZ Wang, XJ Zhang… - Bioresource …, 2018 - Elsevier
Abstract tert-Butyl (3R, 5S)-6-chloro-3, 5-dihydroxyhexanoate ((3R, 5S)-CDHH) is the key
intermediate for synthesis of atorvastatin and rosuvastatin. Carbonyl reductase exhibits …
intermediate for synthesis of atorvastatin and rosuvastatin. Carbonyl reductase exhibits …
Structural‐guided design to improve the catalytic performance of aldo‐keto reductase KdAKR
C Dai, HX Cao, JX Tian, YC Gao, HT Liu… - Biotechnology and …, 2023 - Wiley Online Library
Aldo‐keto reductases (AKRs) are important biocatalysts that can be used to synthesize
chiral pharmaceutical alcohols. In this study, the catalytic activity and stereoselectivity of a …
chiral pharmaceutical alcohols. In this study, the catalytic activity and stereoselectivity of a …
Engineering a Medium‐chain Alcohol Dehydrogenase for Efficient Synthesis of (S)‐N−Boc‐3−pyrrolidinol by Adjusting the Conformational Dynamics of Loops
W Ye, Y Zhang, Y Wang, J Xie, Y Liu, L Yang… - …, 2023 - Wiley Online Library
Loops typically allosterically communicate with active sites, and their conformational
dynamics can affect the catalytic properties of enzymes. Herein, by manipulating the loop …
dynamics can affect the catalytic properties of enzymes. Herein, by manipulating the loop …
Stairway to Stereoisomers: Engineering Short‐and Medium‐Chain Ketoreductases To Produce Chiral Alcohols
AP Shanbhag - ChemBioChem, 2023 - Wiley Online Library
The short‐and medium‐chain dehydrogenase/reductase superfamilies are responsible for
most chiral alcohol production in laboratories and industries. In nature, they participate in …
most chiral alcohol production in laboratories and industries. In nature, they participate in …
Immobilization of recombinant Escherichia coli whole cells harboring xylose reductase and glucose dehydrogenase for xylitol production from xylose mother liquor
LQ Jin, B Yang, W Xu, XX Chen, DX Jia, ZQ Liu… - Bioresource …, 2019 - Elsevier
In this study, recombinant E. coli BL21 (DE3)/pCDFDuet-1-XR-GDH harboring xylose
reductase (XR) and glucose dehydrogenase (GDH) were immobilized and applied for the …
reductase (XR) and glucose dehydrogenase (GDH) were immobilized and applied for the …
Significant improvement of the nitrilase activity by semi-rational protein engineering and its application in the production of iminodiacetic acid
ZQ Liu, MM Lu, XH Zhang, F Cheng, JM Xu… - International journal of …, 2018 - Elsevier
Iminodiacetic acid (IDA) is widely used as an intermediate in the manufacturing of chelating
agents, glyphosate herbicides and surfactants. To improve activity and tolerance to the …
agents, glyphosate herbicides and surfactants. To improve activity and tolerance to the …
Production and characterization of a novel acidophilic and thermostable xylanase from Thermoascus aurantiacu
L Ping, M Wang, X Yuan, F Cui, D Huang, W Sun… - International journal of …, 2018 - Elsevier
The thermostable fungus, Thermoascus aurantiacus M-2, which produces a novel
acidophilic and thermostable xylanase was isolated and identified based on its morphology …
acidophilic and thermostable xylanase was isolated and identified based on its morphology …