Horizontal gene transfer (HGT) is arguably the most conspicuous feature of bacterial
evolution. Evidence for HGT is found in most bacterial genomes. Although HGT can …

It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen and a model
bacterium for studying virulence and bacterial social traits. While it can be isolated in low …

Essential genes refer to genes that are required by an organism to survive under specific
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …

Pseudomonas aeruginosa is an opportunistic pathogen that synthesizes and secretes a
wide range of virulence factors. P. aeruginosa poses a potential threat to human health …

The pangenome refers to a collection of genomic sequence found in the entire species or
population rather than in a single individual; the sequence can be core, present in all …

Pseudomonas aeruginosa is a ubiquitous, motile, gram-negative bacterium that has been
recently identified as a multi-drug resistant pathogen in critical need of novel therapeutics. Of …

Many bacterial species are represented by a pan-genome, whose genetic repertoire far
outstrips that of any single bacterial genome. Here we investigate how a bacterial pan …

Bacteria from the same species can differ widely in their gene content. In Escherichia coli,
the set of genes shared by all strains, known as the core genome, represents about half the …

Fungal pathogens pose a global threat to human health, with Candida albicans among the
leading killers. Systematic analysis of essential genes provides a powerful strategy to …