A synthetic lethal interaction occurs between two genes when the perturbation of either gene
alone is viable but the perturbation of both genes simultaneously results in the loss of …

The genetic concept of synthetic lethality has now been validated clinically through the
demonstrated efficacy of poly (ADP-ribose) polymerase (PARP) inhibitors for the treatment of …

Although single-gene perturbation screens have revealed a number of new targets,
vulnerabilities specific to frequently altered drivers have not been uncovered. An important …

Naturally evolved organisms typically have large genomes that enable their survival and
growth under various conditions. However, the complexity of genomes often precludes our …

Although the genes essential for life have been identified in less complex model organisms,
their elucidation in human cells has been hindered by technical barriers. We used extensive …

Saccharomyces cerevisiae is an increasingly attractive host for synthetic biology because of
its long history in industrial fermentations. However, until recently, most synthetic biology …

Next-generation DNA sequencing technologies have led to a massive accumulation of
genomic and transcriptomic data from patients and healthy individuals. The major challenge …

Large-scale genetic analysis of lethal phenotypes has elucidated the molecular
underpinnings of many biological processes. Using the bacterial clustered regularly …

INTRODUCTION Genetic interactions occur when mutations in different genes combine to
result in a phenotype that is different from expectation based on those of the individual …

Seminal yeast studies have established the value of comprehensively mapping genetic
interactions (GIs) for inferring gene function. Efforts in human cells using focused gene sets …