Dissecting specific and global transcriptional regulation of bacterial gene expression
Gene expression is regulated by specific transcriptional circuits but also by the global
expression machinery as a function of growth. Simultaneous specific and global regulation …
expression machinery as a function of growth. Simultaneous specific and global regulation …
Design of four small-molecule-inducible systems in the yeast chromosome, applied to optimize terpene biosynthesis
The optimization of cellular functions often requires the balancing of gene expression, but
the physical construction and screening of alternative designs are costly and time …
the physical construction and screening of alternative designs are costly and time …
Metabolome and proteome analyses reveal transcriptional misregulation in glycolysis of engineered E. coli
Synthetic metabolic pathways are a burden for engineered bacteria, but the underlying
mechanisms often remain elusive. Here we show that the misregulated activity of the …
mechanisms often remain elusive. Here we show that the misregulated activity of the …
Indirect and suboptimal control of gene expression is widespread in bacteria
Gene regulation in bacteria is usually described as an adaptive response to an
environmental change so that genes are expressed when they are required. We instead …
environmental change so that genes are expressed when they are required. We instead …
Engineering plant metabolism into microbes: from systems biology to synthetic biology
Plant metabolism represents an enormous repository of compounds that are of
pharmaceutical and biotechnological importance. Engineering plant metabolism into …
pharmaceutical and biotechnological importance. Engineering plant metabolism into …
Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories
To overcome environmental problems caused by the use of fossil resources, microbial cell
factories have become a promising technique for the sustainable and eco-friendly …
factories have become a promising technique for the sustainable and eco-friendly …
Metabolic feedback circuits provide rapid control of metabolite dynamics
Metabolism constitutes the basis of life, and the dynamics of metabolism dictate various
cellular processes. However, exactly how metabolite dynamics are controlled remains …
cellular processes. However, exactly how metabolite dynamics are controlled remains …
Trade-offs in biosensor optimization for dynamic pathway engineering
Recent progress in synthetic biology allows the construction of dynamic control circuits for
metabolic engineering. This technology promises to overcome many challenges …
metabolic engineering. This technology promises to overcome many challenges …
Computing the functional proteome: recent progress and future prospects for genome-scale models
EJ O'Brien, BO Palsson - Current opinion in biotechnology, 2015 - Elsevier
Highlights•Models of metabolism and gene expression (ME-Models) now exist for model
organisms.•ME-Models account for∼ 80% of the proteome mass.•ME-Models enable …
organisms.•ME-Models account for∼ 80% of the proteome mass.•ME-Models enable …
Synthetic gene circuits for metabolic control: design trade-offs and constraints
DA Oyarzún, GBV Stan - Journal of the Royal Society …, 2013 - royalsocietypublishing.org
A grand challenge in synthetic biology is to push the design of biomolecular circuits from
purely genetic constructs towards systems that interface different levels of the cellular …
purely genetic constructs towards systems that interface different levels of the cellular …