The nucleic acid therapeutics field has made tremendous progress in the past decades.
Continuous advances in chemistry and design have led to many successful clinical …

Nucleic acids (DNA and RNA) dynamically fold and unfold to exert their functions in cells.
These folding and unfolding behaviours are also the basis for various technical applications …

Oligonucleotide gene therapy (OGT) agents (eg antisense, deoxyribozymes, siRNA and
CRISPR/Cas) are promising therapeutic tools. Despite extensive efforts, only few OGT drugs …

Off-target interactions between antisense oligonucleotides (ASOs) with state-of-the-art
modifications and biological components still pose clinical safety liabilities. To mitigate a …

Steric-blocking oligonucleotides (SBOs) are short, single-stranded nucleic acids designed to
modulate gene expression by binding to RNA transcripts and blocking access from cellular …

Single-stranded regions of RNA are important for folding of sequences into 3D structures
and for design of therapeutics targeting RNA. Prediction of ensembles of 3D structures for …

Abstract The stability of Watson–Crick paired RNA/DNA hybrids is important for designing
optimal oligonucleotides for ASO (Antisense Oligonucleotide) and CRISPR (Clustered …

The intracellular environment is crowded and heterogeneous. Although the thermodynamic
stability of nucleic acid duplexes is predictable in dilute solutions, methods of predicting …

During the last decade, therapeutic oligonucleotide drugs (OND) have witnessed a
tremendous development in chemistry and mechanistic understanding that have translated …

Background The field of antisense oligonucleotide therapeutics is rapidly growing and in
addition to small molecules and therapeutic antibodies, oligonucleotide-based gene …