Engineering the plastid genome based on homologous recombination is well developed in
a few model species. Homologous recombination is also the rule in mitochondria, but …

Genome editing techniques are being used to modify plant breeding, which might increase
food production sustainably by 2050. A product made feasible by genome editing is …

Mutations with deleterious consequences in nature may be conditionally deleterious in crop
plants. That is, while some genetic variants may reduce fitness under wild conditions and be …

Attenuated strains of the naturally occurring plant pathogen Agrobacterium tumefaciens can
transfer virtually any DNA sequence of interest to model plants and crops. This has made …

Spontaneous chromosomal rearrangements (CRs) play an essential role in speciation,
genome evolution and crop domestication. To be able to use the potential of CRs for …

Recent advancements in gene editing provide unprecedented tools for plant biology
research and offer unlimited potential for improving existing crops and de novo …

Non‐homologous end joining (NHEJ) plays a major role in repairing DNA double‐strand
breaks and is key to genome stability and editing. The minimal core NHEJ proteins, namely …

Gene targeting can be used to make modifications at a specific region in a plant's genome
and create high-precision tools for plant biotechnology and breeding. However, its low …

The CRISPR-Cas9 system has been deployed for precision mutagenesis in an ever-growing
number of species, including agricultural crops and forest trees. Its application to closely …

The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is a protein encoded by
the PRKDC gene in humans and plays a crucial role in repairing DNA double-strand breaks …