The recruitment model for gene activation stipulates that an activator works by bringing the
transcriptional machinery to the DNA. Recent experiments in bacteria and yeast indicate that …

Transcription activation by Escherichia coli catabolite activator protein (CAP) at each of two
classes of simple CAP-dependent promoters is understood in structural and mechanistic …

During transcription initiation, RNA polymerase (RNAP) binds and unwinds promoter DNA to
form an RNAP-promoter open complex. We have determined crystal structures at 2.9 and …

Recently determined structures of the Escherichia coli catabolite activator protein (CAP) in
complex with DNA, and in complex with the RNA polymerase α subunit C-terminal domain …

The β-amyloid precursor protein (βAPP) gene of the mouse was disrupted by inserting into
exon 2 a cassette containing a neomycin resistance gene and a putative transcription …

Drug-resistant bacterial pathogens pose an urgent public-health crisis. Here, we report the
discovery, from microbial-extract screening, of a nucleoside-analog inhibitor that inhibits …

The synthesis of ribosomal RNA is the rate-limiting step in ribosome synthesis in bacteria.
There are multiple mechanisms that determine the rate of rRNA synthesis. Ribosomal RNA …

Many transcriptional activators in prokaryotes are known to bind near a promoter and
contact RNA polymerase1–5, but it is not clear whether a protein–protein contact between …

In recent years, it has become clear that promoter recognition by bacterial RNA polymerase
involves interactions not only between core promoter elements and the σ subunit, but also …

We have used systematic fluorescence resonance energy transfer and distance-constrained
docking to define the three-dimensional structures of bacterial RNA polymerase holoenzyme …