Targeting topoisomerase I: molecular mechanisms and cellular determinants of response to topoisomerase I inhibitors
GL Beretta, P Perego, F Zunino - Expert opinion on therapeutic …, 2008 - Taylor & Francis
Background: Topoisomerase I is required for DNA relaxation during critical cellular
functions. The identification of camptothecins as specific enzyme inhibitors and their clinical …
functions. The identification of camptothecins as specific enzyme inhibitors and their clinical …
Laser scanning cytometry
LA Kamentsky - Methods in cell biology, 2001 - Elsevier
Publisher Summary Laser scanning cytometry (LSCM) automatically measures laser excited
fluorescence at multiple wavelengths and light scatter from cells on slides that have been …
fluorescence at multiple wavelengths and light scatter from cells on slides that have been …
[HTML][HTML] Purinergic signaling regulates neural progenitor cell expansion and neurogenesis
JHC Lin, T Takano, G Arcuino, X Wang, F Hu… - Developmental …, 2007 - Elsevier
Neural stem and progenitor cells typically exhibit a density-dependent survival and
expansion, such that critical densities are required below which clonogenic progenitors are …
expansion, such that critical densities are required below which clonogenic progenitors are …
[HTML][HTML] A potential role of nuclear matrix-associated protein kinase CK2 in protection against drug-induced apoptosis in cancer cells
Protein kinase CK2 (CK2) has long been implicated in the regulation of cell growth and
proliferation. Its activity is generally elevated in rapidly proliferating tissues, and nuclear …
proliferation. Its activity is generally elevated in rapidly proliferating tissues, and nuclear …
DNA damage induced by DNA topoisomerase I-and topoisomerase II-inhibitors detected by histone H2AXphosphorylation in relation to the cell cycle phase and …
X Huang, F Traganos, Z Darzynkiewicz - Cell Cycle, 2003 - Taylor & Francis
Histone H2AX is phosphorylated on Ser-139 by ATM kinase in response to damage that
induces dsDNA breaks. Immunocytochemical detection of phosphorylated H2AX (gH2AX) …
induces dsDNA breaks. Immunocytochemical detection of phosphorylated H2AX (gH2AX) …
Cleavage of poly (ADP-ribose) polymerase measured in situ in individual cells: relationship to DNA fragmentation and cell cycle position during apoptosis
X Li, Z Darzynkiewicz - Experimental cell research, 2000 - Elsevier
Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA repair, is a target
of caspases during apoptosis: its cleavage onto 89-and 24-kDa fragments is considered to …
of caspases during apoptosis: its cleavage onto 89-and 24-kDa fragments is considered to …
Synchronization in the cell cycle by inhibitors of DNA replication induces histone H2AX phosphorylation: an indication of DNA damage
A Kurose, T Tanaka, X Huang, F Traganos… - Cell …, 2006 - Wiley Online Library
Several methods to synchronize cultured cells in the cell cycle are based on temporary
inhibition of DNA replication. Previously it has been reported that cells synchronized this …
inhibition of DNA replication. Previously it has been reported that cells synchronized this …
Laser scanning cytometry: principles and applications—an update
P Pozarowski, E Holden, Z Darzynkiewicz - Cell Imaging Techniques …, 2013 - Springer
Laser scanning cytometer (LSC) is the microscope-based cytofluorometer that offers a
plethora of unique analytical capabilities, not provided by flow cytometry (FCM). This review …
plethora of unique analytical capabilities, not provided by flow cytometry (FCM). This review …
Translocation of Bax to mitochondria during apoptosis measured by laser scanning cytometry
E Bedner, X Li, J Kunicki… - Cytometry: The Journal …, 2000 - Wiley Online Library
Background During induction of apoptosis, the pro‐apoptotic member of the Bcl‐2 protein
family (Bax) undergoes translocation to the mitochondria. The translocation, which leads to …
family (Bax) undergoes translocation to the mitochondria. The translocation, which leads to …
New molecular mechanisms of action of camptothecin-type drugs
K Legarza, LX Yang - Anticancer research, 2006 - ar.iiarjournals.org
Camptothecin (CPT) derivatives have emerged as a promising group of chemotherapeutic
agents. The FDA has approved the CPT derivatives topotecan and irinotecan for second line …
agents. The FDA has approved the CPT derivatives topotecan and irinotecan for second line …