The RAG proteins and V (D) J recombination: complexes, ends, and transposition
SD Fugmann, AI Lee, PE Shockett… - Annual review of …, 2000 - annualreviews.org
V (D) J recombination proceeds through a series of protein: DNA complexes mediated in
part by the RAG1 and RAG2 proteins. These proteins are responsible for sequence-specific …
part by the RAG1 and RAG2 proteins. These proteins are responsible for sequence-specific …
Recruitment of CRISPR-Cas systems by Tn7-like transposons
JE Peters, KS Makarova… - Proceedings of the …, 2017 - National Acad Sciences
A survey of bacterial and archaeal genomes shows that many Tn7-like transposons contain
minimal type IF CRISPR-Cas systems that consist of fused cas8f and cas5f, cas7f, and cas6f …
minimal type IF CRISPR-Cas systems that consist of fused cas8f and cas5f, cas7f, and cas6f …
[HTML][HTML] Structures of the holo CRISPR RNA-guided transposon integration complex
CRISPR-associated transposons (CAST) are programmable mobile genetic elements that
insert large DNA cargos using an RNA-guided mechanism,–. CAST elements contain …
insert large DNA cargos using an RNA-guided mechanism,–. CAST elements contain …
[HTML][HTML] Tn5 in vitro transposition
IY Goryshin, WS Reznikoff - Journal of Biological Chemistry, 1998 - ASBMB
This communication reports the development of an efficient in vitro transposition system for
Tn5. A key component of this system was the use of hyperactive mutant transposase. The …
Tn5. A key component of this system was the use of hyperactive mutant transposase. The …
[PDF][PDF] Structural basis of transposon end recognition explains central features of Tn7 transposition systems
Z Kaczmarska, M Czarnocki-Cieciura… - Molecular cell, 2022 - cell.com
Tn7 is a bacterial transposon with relatives containing element-encoded CRISPR-Cas
systems mediating RNA-guided transposon insertion. Here, we present the 2.7 Å …
systems mediating RNA-guided transposon insertion. Here, we present the 2.7 Å …
IntI2 Integron Integrase in Tn7
K Hansson, L Sundström, A Pelletier… - Journal of …, 2002 - Am Soc Microbiol
Integrons can insert and excise antibiotic resistance genes on plasmids in bacteria by site-
specific recombination. Class 1 integrons code for an integrase, IntI1 (337 amino acids in …
specific recombination. Class 1 integrons code for an integrase, IntI1 (337 amino acids in …
The Tn7 transposase is a heteromeric complex in which DNA breakage and joining activities are distributed between different gene products.
RJ Sarnovsky, EW May, NL Craig - The EMBO Journal, 1996 - embopress.org
The bacterial transposon Tn7 translocates by a cut and paste mechanism: excision from the
donor site results from double‐strand breaks at each end of Tn7 and target insertion results …
donor site results from double‐strand breaks at each end of Tn7 and target insertion results …
[PDF][PDF] Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex
Abstract During V (D) J recombination, the RAG1 and RAG2 proteins cooperate to catalyze a
series of DNA bond breakage and strand transfer reactions. The structure, location, and …
series of DNA bond breakage and strand transfer reactions. The structure, location, and …
Drosophila P-element transposase is a novel site-specific endonuclease
EL Beall, DC Rio - Genes & development, 1997 - genesdev.cshlp.org
We developed in vitro assays to study the first step of the P-element transposition reaction:
donor DNA cleavage. We found that P-element transposase required both 5′ and 3′ P …
donor DNA cleavage. We found that P-element transposase required both 5′ and 3′ P …