P450-driven plastic-degrading synthetic bacteria

SJ Yeom, TK Le, CH Yun - Trends in Biotechnology, 2022 - cell.com
Plastic contamination currently threatens a wide variety of ecosystems and presents
damaging repercussions and negative consequences for many wildlife species. Sustainable …

Hemoprotein catalyzed oxygenations: P450s, UPOs, and progress toward scalable reactions

G Grogan - JACS Au, 2021 - ACS Publications
The selective oxygenation of nonactivated carbon atoms is an ongoing synthetic challenge,
and biocatalysts, particularly hemoprotein oxygenases, continue to be investigated for their …

Evaluation of P450 monooxygenase activity in lyophilized recombinant E. coli cells compared to resting cells

T Hilberath, A Raffaele, LM Windeln, VB Urlacher - AMB Express, 2021 - Springer
Cytochromes P450 catalyze oxidation of chemically diverse compounds and thus offer great
potential for biocatalysis. Due to the complexity of these enzymes, their dependency of …

Development of a Bacterial FhuD-Lysozyme-SsrA Mediated Autolytic (FLSA) System for Effective Release of Intracellular Products

F Zhang, X Fan, K Xu, S Wang, S Shi, L Yi… - ACS Synthetic …, 2022 - ACS Publications
Developing effective bacterial autolytic systems for fast release of intracellular bioproducts
could simplify purification procedures and help with the high throughput screening of mutant …

Use of Copper as a Trigger for the in Vivo Activity of E. coli Laccase CueO: A Simple Tool for Biosynthetic Purposes

D Decembrino, M Girhard, VB Urlacher - ChemBioChem, 2021 - Wiley Online Library
Laccases are multi‐copper oxidases that catalyze the oxidation of various electron‐rich
substrates with concomitant reduction of molecular oxygen to water. The multi‐copper …

Investigation of Vitamin D2 and Vitamin D3 Hydroxylation by Kutzneria albida

LM Schmitz, A Kinner, K Althoff, K Rosenthal… - …, 2021 - Wiley Online Library
The active vitamin D metabolites 25‐OH− D and 1α, 25‐(OH) 2− D play an essential role in
controlling several cellular processes in the human body and are potentially effective in the …

Deconstruction of the CYP153A6 alkane hydroxylase system: limitations and optimization of in vitro alkane hydroxylation

S Kochius, J Van Marwijk, AC Ebrecht, DJ Opperman… - Catalysts, 2018 - mdpi.com
Some of the most promising results for bacterial alkane hydroxylation to alcohols have been
obtained with the cytochrome P450 monooxygenase CYP153A6. CYP153A6 belongs to the …

Chaperone-mediated protein folding enhanced D-psicose 3-epimerase expression in engineered Bacillus subtilis

J Chen, H Wei, Y Guo, Q Li, H Wang, J Liu - Process Biochemistry, 2021 - Elsevier
Functional mature proteins usually undergo folding, and in vivo folding is often affected by
factors such as translation ratio, intracellular environment, and chaperones. d-psicose 3 …

Butylbenzene and tert-Butylbenzene—Sorption on Sand Particles and Biodegradation in the Presence of Plant Natural Surfactants

A Zdarta, A Pacholak, M Galikowska, W Smułek… - Toxins, 2018 - mdpi.com
The effects of hydrocarbons sorption on sand and saponins presence in the system on
butylbenzene and tert-butylbenzene biological degradation was investigated. Additionally …

Oxygen transfer in cell-free and simulated alkane-based bioprocesses: Computational Fluid Dynamics (CFD) simulations and predictive modelling

GK Gakingo - 2020 - scholar.sun.ac.za
Alkane activation using whole-cell biocatalysts is an attractive technology that can be
employed in alkane valorisation. However, since metabolically active cells involved in this …