The rapid increase in the number of diabetic patients globally and exploration of alternate
insulin delivery methods such as inhalation or oral route that rely on higher doses, is bound …

Escherichia coli is the most preferred microorganism to express heterologous proteins for
therapeutic use, as around 30% of the approved therapeutic proteins are currently being …

The past 20 years have seen enormous progress in the understanding of the mechanisms
used by the enteric bacterium Escherichia coli to promote protein folding, support protein …

Aging, defined as a decrease in reproduction rate with age, is a fundamental characteristic
of all living organisms down to bacteria. Yet we know little about the causal molecular …

Overexpression of membrane proteins is often essential for structural and functional studies,
but yields are frequently too low. An understanding of the physiological response to …

Protein aggregation is an ordinary consequence of thermal stress. In recombinant bacteria,
the over-expression of plasmid-encoded genes triggers transcription of heat-shock genes …

Different species of microorganisms including yeasts, filamentous fungi and bacteria have
been used in the past 25 years for the controlled production of foreign proteins of scientific …

A common limitation of recombinant protein production in bacteria is the formation of
insoluble protein aggregates known as inclusion bodies. The propensity of a given protein to …

The efficient in vivo folding of many heterologous proteins is a major bottleneck of high level
production in bacterial hosts and simple optimisation protocols have not been available yet …

Microbial enzymes have been used in a large number of fields, such as chemical,
agricultural and biopharmaceutical industries. The enzyme production rate and yield are the …