Fertility preservation for prepubertal boys: lessons learned from the past and update on remaining challenges towards clinical translation
BACKGROUND Childhood cancer incidence and survivorship are both on the rise.
However, many lifesaving treatments threaten the prepubertal testis. Cryopreservation of …
However, many lifesaving treatments threaten the prepubertal testis. Cryopreservation of …
In‐vitro spermatogenesis through testis modelling: Toward the generation of testicular organoids
G Richer, Y Baert, E Goossens - Andrology, 2020 - Wiley Online Library
Background The testicular organoid concept has recently been introduced in tissue
engineering to refer to testicular cell organizations modeling testicular architecture and …
engineering to refer to testicular cell organizations modeling testicular architecture and …
Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis
M Salem, F Khadivi, P Javanbakht… - Stem Cell Research & …, 2023 - Springer
The loss of germ cells and spermatogenic failure in non-obstructive azoospermia are
believed to be the main causes of male infertility. Laboratory studies have used in vitro …
believed to be the main causes of male infertility. Laboratory studies have used in vitro …
What has single-cell RNA-seq taught us about mammalian spermatogenesis?
S Suzuki, VD Diaz, BP Hermann - Biology of Reproduction, 2019 - academic.oup.com
Mammalian spermatogenesis is a complex developmental program that transforms mitotic
testicular germ cells (spermatogonia) into mature male gametes (sperm) for production of …
testicular germ cells (spermatogonia) into mature male gametes (sperm) for production of …
In vitro spermatogenesis: A century‐long research journey, still half way around
M Komeya, T Sato, T Ogawa - Reproductive medicine and …, 2018 - Wiley Online Library
Background Spermatogenesis is one of the most complicated cellular differentiation
processes in a body. Researchers struggled to find and develop a micro‐environmental …
processes in a body. Researchers struggled to find and develop a micro‐environmental …
Human in vitro spermatogenesis as a regenerative therapy—where do we stand?
M Robinson, S Sparanese, L Witherspoon… - Nature Reviews …, 2023 - nature.com
Spermatogenesis involves precise temporal and spatial gene expression and cell signalling
to reach a coordinated balance between self-renewal and differentiation of spermatogonial …
to reach a coordinated balance between self-renewal and differentiation of spermatogonial …
Stem cells as a resource for treatment of infertility-related diseases
J Wang, C Liu, M Fujino, G Tong… - Current molecular …, 2019 - ingentaconnect.com
Worldwide, infertility affects 8-12% of couples of reproductive age and has become a
common problem. There are many ways to treat infertility, including medication, intrauterine …
common problem. There are many ways to treat infertility, including medication, intrauterine …
Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control
T Matsumura, T Sato, T Abe, H Sanjo, K Katagiri… - Scientific Reports, 2021 - nature.com
In vitro spermatogenesis (IVS) using air–liquid interphase organ culture method is possible
with mouse testis tissues. The same method, however, has been hardly applicable to …
with mouse testis tissues. The same method, however, has been hardly applicable to …
Three-dimensional testicular organoids as novel in vitro models of testicular biology and toxicology
S Sakib, A Voigt, T Goldsmith… - Environmental …, 2019 - academic.oup.com
Organoids are three dimensional structures consisting of multiple cell types that recapitulate
the cellular architecture and functionality of native organs. Over the last decade, the advent …
the cellular architecture and functionality of native organs. Over the last decade, the advent …
Three-dimensional co-culture of human spermatogonial stem cells with Sertoli cells in soft agar culture system supplemented by growth factors and Laminin
Application of a three-dimensional (3D) culture system for in vitro proliferation and
differentiation of human spermatogonial stem cells (SSCs) is a useful tool for the …
differentiation of human spermatogonial stem cells (SSCs) is a useful tool for the …