The aptamer field witnessed steady growth during the past 28 years as evident from the
exponentially increasing number of related publications. The field is “coming of age”, but like …

Melting temperatures, T m, were systematically studied for a set of 92 DNA duplex oligomers
in a variety of sodium ion concentrations ranging from 69 mM to 1.02 M. The relationship …

DNA and RNA, the nucleic acids found in every living organism, are quite crucial, because
not only do they store the genetic information, but also they are used as signals through …

Molecular beacons are sensitive fluorescent probes hybridizing selectively to designated
DNA and RNA targets. They have recently become practical tools for quantitative real-time …

ABSTRACT RNA research and applications are underpinned by in vitro transcription (IVT),
but RNA impurities resulting from the enzymatic reagents severely impede downstream …

Methyl-cytosine (5mC) is one of the most important DNA modifications and plays versatile
biological roles. It is well known that 5mC stabilizes DNA duplexes. However, it remains …

Aptamers are a unique class of nucleic acid molecules currently being investigated for
clinical applications. Aptamers are functional single-stranded oligonucleotides able to form …

Several different pathways, generally termed RNA silencing pathways, utilize small RNA
molecules guiding sequence-specific silencing effects of ribonucleoprotein effector …

The substitution of 2′-fluoro for 2′-hydroxyl moieties in RNA substantially improves the
stability of RNA. RNA stability is a major issue in RNA research and applications involving …

In recent years modified oligonucleotides (oligos) have become an area of increased
research activity. These synthetic biopolymers have long been used as molecular probes to …