Over the last three decades, the development of new genome editing techniques, such as
ODM, TALENs, ZFNs and the CRISPR‐Cas system, has led to significant progress in the …

Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion
from today. To meet the demands of growing, geographically and socio-economically …

CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …

Summary Recently, CRISPR‐Cas12a (Cpf1) from Prevotella and Francisella was
engineered to modify plant genomes. In this report, we employed CRISPR‐LbCas12a …

Engineering CRISPR/LbCas12a for highly efficient, temperature‐tolerant plant gene editing -
PMC Back to Top Skip to main content NIH NLM Logo Access keys NCBI Homepage MyNCBI …

Remarkable diversity in the domain of genome loci architecture, structure of effector
complex, array of protein composition, mechanisms of adaptation along with difference in …

The CRISPR/Cas system comprises RNA-guided nucleases, the target specificity of which is
directed by Watson–Crick base pairing of target loci with single guide (sg) RNA to induce the …

Cpf1, an endonuclease of the class 2 CRISPR family, fills the gaps that were previously
faced in the world of genome engineering tools, which include the TALEN, ZFN, and …

Contamination of agricultural land and water by heavy metals due to rapid industrialization
and urbanization including various natural processes have become one of the major …

As an allotetraploid, most genes have multiple copies that belong to At and Dt subgenomes
in cotton (Gossypium hirsutum). Allotetraploid cotton genome is very complex (AADD, 2n …