Scanning transmission electron microscopy (STEM) has emerged as a uniquely powerful
tool for structural and functional imaging of materials on the atomic level. Driven by …

Life exists in three dimensions, but until the turn of the century most electron microscopy
methods provided only 2D image data. Recently, electron microscopy techniques capable of …

We describe new tools for the processing of electron cryo-microscopy (cryo-EM) images in
the fourth major release of the RELION software. In particular, we introduce VDAM, a …

The EMBL-EBI search and sequence analysis tools frameworks provide integrated access to
EMBL-EBI's data resources and core bioinformatics analytical tools. EBI Search …

Cryo-electron microscopy (cryo-EM) single-particle analysis has proven powerful in
determining the structures of rigid macromolecules. However, many imaged protein …

Public archiving in structural biology is well established with the Protein Data Bank (PDB;
wwPDB. org) catering for atomic models and the Electron Microscopy Data Bank (EMDB; …

Methods are presented that detect three types of aberrations in single-particle cryo-EM data
sets: symmetrical and antisymmetrical optical aberrations and magnification anisotropy …

Here, we describe the third major release of RELION. CPU-based vector acceleration has
been added in addition to GPU support, which provides flexibility in use of resources and …

Cryogenic electron tomography (cryoET) allows visualization of cellular structures in situ.
However, anisotropic resolution arising from the intrinsic “missing-wedge” problem has …

The acquisition of cryo-electron microscopy (cryo-EM) data from biological specimens must
be tightly coupled to data preprocessing to ensure the best data quality and microscope …