Microspore embryogenesis: targeting the determinant factors of stress-induced cell reprogramming for crop improvement

PS Testillano - Journal of Experimental Botany, 2019 - academic.oup.com
Under stress, isolated microspores are reprogrammed in vitro towards embryogenesis,
producing doubled haploid plants that are useful biotechnological tools in plant breeding as …

Improving the efficiency of wheat microspore culture methodology: Evaluation of pretreatments, gradients, and epigenetic chemicals

HM Wang, JL Enns, KL Nelson, JM Brost… - Plant Cell, Tissue and …, 2019 - Springer
The objective of the study was to develop efficient widely adaptable microspore culture
methodology for spring and winter wheat genotypes. Current wheat microspore culture …

Trichostatin A affects developmental reprogramming of bread wheat microspores towards an embryogenic route

AM Castillo, I Valero-Rubira, MÁ Burrell, S Allué… - Plants, 2020 - mdpi.com
Microspores can be developmentally reprogrammed by the application of different stress
treatments to initiate an embryogenic pathway leading to the production of doubled haploid …

Live Imaging of embryogenic structures in Brassica napus microspore embryo cultures highlights the developmental plasticity of induced totipotent cells

P Corral-Martínez, C Siemons, A Horstman… - Plant reproduction, 2020 - Springer
Key message In vitro embryo development is highly plastic; embryo cell fate can be re-
established in tissue culture through different pathways. Abstract In most angiosperms …

Microspore embryogenesis induction by mannitol and TSA results in a complex regulation of epigenetic dynamics and gene expression in bread wheat

I Valero-Rubira, AM Castillo, MÁ Burrell… - Frontiers in Plant …, 2023 - frontiersin.org
Reprogramming of microspores development towards embryogenesis mediated by stress
treatment constitutes the basis of doubled haploid production. Recently, compounds that …

Trichostatin A and sodium butyrate promotes plant regeneration in common wheat

XM Bie, L Dong, XH Li, H Wang, XQ Gao… - Plant Signaling & …, 2020 - Taylor & Francis
Histone acetylation modification plays a vital role in plant cell division and differentiation.
However, the function on wheat mature embryo culture has not been reported. Here, we …

The roads to haploid embryogenesis

K Shen, M Qu, P Zhao - Plants, 2023 - mdpi.com
Although zygotic embryogenesis is usually studied in the field of seed biology, great
attention has been paid to the methods used to generate haploid embryos due to their …

Site-directed mutagenesis in barley using RNA-guided Cas endonucleases during microspore-derived generation of doubled haploids

RE Hoffie, I Otto, H Hisano, J Kumlehn - Doubled Haploid Technology …, 2021 - Springer
In plant research and breeding, haploid technology is employed upon crossing, induced
mutagenesis or genetic engineering to generate populations of meiotic recombinants that …

The different response of Brassica napus genotypes to microspore embryogenesis induced by heat shock and trichostatin A is not determined by changes in cell wall …

C Camacho‐Fernández, P Corral‐Martínez… - Physiologia …, 2024 - Wiley Online Library
During microspore embryogenesis, microspores are induced to develop into haploid
embryos. In Brassica napus, microspore embryogenesis is induced by a heat shock (HS) …

New Epigenetic Modifier Inhibitors Enhance Microspore Embryogenesis in Bread Wheat

I Valero-Rubira, MP Vallés, B Echávarri, P Fustero… - Plants, 2024 - mdpi.com
The use of doubled haploid (DH) technology enables the development of new varieties of
plants in less time than traditional breeding methods. In microspore embryogenesis (ME) …