With rapid advances in understanding molecular pathogenesis of human diseases in the era
of genome sciences and systems biology, it is anticipated that increasing numbers of …

Uses of viral vectors have thus far eclipsed uses of non-viral vectors for gene therapy
delivery in the clinic. Viral vectors, however, have certain issues involving genome …

Mammalian cell expression has become the dominant recombinant protein production
system for clinical applications because of its capacity for post-translational modification and …

A scalable transfection procedure using polyethylenimine (PEI) is described for the human
embryonic kidney 293 cell line grown in suspension. Green fluorescent protein (GFP) and …

Secreted mammalian proteins require the development of robust protein over‐expression
systems for crystallographic and biophysical studies of protein function. Due to complex …

Genetic reprogramming of somatic cells to a pluripotent state (induced pluripotent stem cells
or iPSCs) by over-expression of specific genes has been accomplished using mouse and …

Biological mass spectrometry (MS) encompasses a range of methods for characterizing
proteins and other biomolecules. MS is uniquely powerful for the structural analysis of …

The expansion of the biologics pipeline depends on the identification of candidate proteins
for clinical trials. Speed is one of the critical issues, and the rapid production of high quality …

Switching mechanisms that control genes could be viewed either as stable binary switches,
in which genes exist in'on'or'off'states; or as quantitative rheostat-like switches, in which the …

Efficient strategies for the production of recombinant proteins are gaining increasing
importance, as more applications that require high amounts of high-quality proteins reach …