Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Spreading depression (SD) is a transient wave of near-complete neuronal and glial
depolarization associated with massive transmembrane ionic and water shifts. It is …

Although modern techniques such as two-photon microscopy can now provide cellular-level
three-dimensional imaging of the intact living brain, the speed and fields of view of these …

Imaging techniques based on optical contrast analysis can be used to visualize dynamic
and functional properties of the nervous system via optical signals resulting from changes in …

Optical fibers guide light between separate locations and enable new types of fluorescence
imaging. Fiber-optic fluorescence imaging systems include portable handheld microscopes …

Optical microscopic imaging offers opportunities to perform noninvasive assessments of
numerous parameters associated with the biochemistry, morphology, and functional state of …

Background & Aims: The aim of this study was to assess the potential of 3 spectroscopic
techniques (fluorescence, reflectance, and light-scattering spectroscopy) individually and in …

Reactive oxygen species (ROS) formation and signaling are of major importance and
regulate a number of processes in physiological conditions. A disruption in redox status …

Normal mitochondrial function is a critical factor in maintaining cellular homeostasis in
various organs of the body. Due to the involvement of mitochondrial dysfunction in many …

During the development of neoplasia, epithelial tissues undergo biochemical and structural
changes that can manifest in tissue fluorescence. There have been several reports on …